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タイトル: | Activation-induced cytidine deaminase alters the subcellular localization of tet family proteins. |
著者: | Arioka, Yuko Watanabe, Akira ![]() ![]() Saito, Kuniaki Yamada, Yasuhiro ![]() |
著者名の別形: | 山田, 泰広 |
発行日: | 17-Sep-2012 |
出版者: | Public Library of Science |
誌名: | PloS one |
巻: | 7 |
号: | 9 |
論文番号: | e45031 |
抄録: | Activation-induced cytidine deminase (Aid), a unique enzyme that deaminates cytosine in DNA, shuttles between the nucleus and the cytoplasm. A recent study proposed a novel function of Aid in active DNA demethylation via deamination of 5-hydroxymethylcytosine, which is converted from 5-methylcytosine by the Ten-eleven translocation (Tet) family of enzymes. In this study, we examined the effect of simultaneous expression of Aid and Tet family proteins on the subcellular localization of each protein. We found that overexpressed Aid is mainly localized in the cytoplasm, whereas Tet1 and Tet2 are localized in the nucleus, and Tet3 is localized in both the cytoplasm and the nucleus. However, nuclear Tet proteins were gradually translocated to the cytoplasm when co-expressed with Aid. We also show that Aid-mediated translocation of Tet proteins is associated with Aid shuttling. Here we propose a possible role for Aid as a regulator of the subcellular localization of Tet family proteins. |
著作権等: | © 2012 Arioka et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
URI: | http://hdl.handle.net/2433/160364 |
DOI(出版社版): | 10.1371/journal.pone.0045031 |
PubMed ID: | 23028748 |
出現コレクション: | 学術雑誌掲載論文等 |
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