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DCフィールド | 値 | 言語 |
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dc.contributor.author | Tanaka, Rina | en |
dc.contributor.author | Tainaka, Motomi | en |
dc.contributor.author | Ota, Takumi | en |
dc.contributor.author | Mizuguchi, Naoki | en |
dc.contributor.author | Kato, Hiroyuki | en |
dc.contributor.author | Urabe, Shoichi | en |
dc.contributor.author | Chen, Yulin | en |
dc.contributor.author | Fustin, Jean-Michel | en |
dc.contributor.author | Yamaguchi, Yoshiaki | en |
dc.contributor.author | Doi, Masao | en |
dc.contributor.author | Hamada, Shinshichi | en |
dc.contributor.author | Okamura, Hitoshi | en |
dc.contributor.alternative | 岡村, 均 | ja |
dc.date.accessioned | 2012-11-20T05:43:55Z | - |
dc.date.available | 2012-11-20T05:43:55Z | - |
dc.date.issued | 2011-08 | - |
dc.identifier.issn | 0022-1554 | - |
dc.identifier.uri | http://hdl.handle.net/2433/161794 | - |
dc.description.abstract | To ensure the maintenance of tissues in mammals, cell loss must be balanced with cell production, the proliferative activity being different from tissue to tissue. In this article, the authors propose a new method for the quantification of the proliferative activity, defined as the S-phase fraction of actively cycling cells, by dual labeling with fluorescence and peroxidase immunohistochemistry using BrdU (marker of S-phase) and Ki67 antibodies (marker of G(1)-, S-, G(2)-, and M-phases) after a one-step antigen retrieval. In the generative cell zones of fundic and pyloric glandular stomachs, where the majority of cells were cycling, the authors measured a proliferative activity of 31%. In the epithelium of the forestomach and the skin, where cycling cells are intermingled with G(0) and differentiated cells, proliferative activities were 21% and 13%, respectively. In the adrenal cortex, in which cycling cells were sparsely distributed, the proliferative activity reached 32%. During the regenerative process in the skin after a lesion, the proliferative activity increased in proximity to the wound. The present one-step dual-labeling method has revealed that the proliferative activity is different between tissues and depends on the physiological or pathological state. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | SAGE Publications | en |
dc.rights | © 2012 by The Histochemical Society. | en |
dc.rights | This is not the published version. Please cite only the published version. | en |
dc.rights | この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 | ja |
dc.subject.mesh | Adrenal Glands/cytology | en |
dc.subject.mesh | Adrenal Glands/metabolism | en |
dc.subject.mesh | Animals | en |
dc.subject.mesh | Antibodies | en |
dc.subject.mesh | Bromodeoxyuridine | en |
dc.subject.mesh | Cell Proliferation | en |
dc.subject.mesh | Gastric Mucosa/cytology | en |
dc.subject.mesh | Gastric Mucosa/metabolism | en |
dc.subject.mesh | Immunohistochemistry | en |
dc.subject.mesh | Indicators and Reagents | en |
dc.subject.mesh | Ki-67 Antigen/metabolism | en |
dc.subject.mesh | Male | en |
dc.subject.mesh | Mice | en |
dc.subject.mesh | Mice, Inbred C57BL | en |
dc.subject.mesh | S Phase | en |
dc.subject.mesh | Skin/cytology | en |
dc.subject.mesh | Skin/injuries | en |
dc.subject.mesh | Skin/metabolism | en |
dc.subject.mesh | Staining and Labeling | en |
dc.subject.mesh | Stomach/cytology | en |
dc.subject.mesh | Stomach/metabolism | en |
dc.subject.mesh | Wound Healing | en |
dc.title | Accurate determination of S-phase fraction in proliferative cells by dual fluorescence and peroxidase immunohistochemistry with 5-bromo-2'-deoxyuridine (BrdU) and Ki67 antibodies. | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.ncid | AA0069926X | - |
dc.identifier.jtitle | The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society | en |
dc.identifier.volume | 59 | - |
dc.identifier.issue | 8 | - |
dc.identifier.spage | 791 | - |
dc.identifier.epage | 798 | - |
dc.relation.doi | 10.1369/0022155411411090 | - |
dc.textversion | author | - |
dc.identifier.pmid | 21551319 | - |
dcterms.accessRights | open access | - |
出現コレクション: | 学術雑誌掲載論文等 |
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