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978-1-61779-998-3_4.pdf | 271.35 kB | Adobe PDF | 見る/開く |
タイトル: | Establishment of the DNA repair-defective mutants in DT40 cells. |
著者: | Ishiai, Masamichi Uchida, Emi Takata, Minoru |
著者名の別形: | 高田, 穣 |
キーワード: | DT40 DNA repair Reverse genetics Gene disruption Conditional targeting |
発行日: | 2012 |
出版者: | Humana Press |
誌名: | Methods in molecular biology |
巻: | 920 |
開始ページ: | 39 |
終了ページ: | 49 |
抄録: | The chicken B cell line DT40 has been widely used as a model system for reverse genetics studies in higher eukaryotes, because of its advantages including efficient gene targeting and ease of chromosome manipulation. Although the genetic approach using the RNA interference technique has become the standard method particularly in human cells, DT40 still remains a powerful tool to investigate the regulation and function of genes and proteins in a vertebrate system, because of feasibility of easy, rapid, and clear genetic experiments. The use of DT40 cells for DNA repair research has several advantages. In addition to canonical assays for DNA repair, such as measurement of the sensitivities toward DNA damage reagents, it is possible to measure homologous recombination and translesion synthesis activities using activation-induced deaminase (AID)-induced diversification of the immunoglobulin locus. In this chapter, we would describe a detailed protocol for gene disruption experiments in DT40 cells. |
著作権等: | The final publication is available at www.springerlink.com This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 |
URI: | http://hdl.handle.net/2433/163156 |
DOI(出版社版): | 10.1007/978-1-61779-998-3_4 |
PubMed ID: | 22941595 |
出現コレクション: | 学術雑誌掲載論文等 |
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