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ファイル | 記述 | サイズ | フォーマット | |
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j.biomaterials.2012.10.059.pdf | 2.95 MB | Adobe PDF | 見る/開く |
タイトル: | The use of fluorescent indoline dyes for side population analysis. |
著者: | Kohara, Hiroshi Watanabe, Kohei Shintou, Taichi Nomoto, Tsuyoshi Okano, Mie Shirai, Tomoaki Miyazaki, Takeshi Tabata, Yasuhiko |
著者名の別形: | 田畑, 泰彦 |
キーワード: | Flow cytometry Side population Fluorescent dye Hematopoietic stem cells |
発行日: | Jan-2013 |
出版者: | Elsevier Ltd. |
誌名: | Biomaterials |
巻: | 34 |
号: | 4 |
開始ページ: | 1024 |
終了ページ: | 1032 |
抄録: | Dye efflux assay evaluated by flow cytometry is useful for stem cell studies. The side population (SP) cells, characterized by the capacity to efflux Hoechst 33342 dye, have been shown to be enriched for hematopoietic stem cells (HSCs) in bone marrow. In addition, SP cells are isolated from various tissues and cell lines, and are also potential candidates for cancer stem cells. However, ultra violet (UV) light, which is not common for every flow cytometer, is required to excite Hoechst 33342. Here we showed that a fluorescent indoline dye ZMB793 can be excited by 488-nm laser, equipped in almost all the modern flow cytometers, and ZMB793-excluding cells showed SP phenotype. HSCs were exclusively enriched in the ZMB793-excluding cells, while ZMB793 was localized in cytosol of bone marrow lineage cells. The efflux of ZMB793 dye was mediated by ATP binding cassette (ABC) transporter Abcg2. Moreover, staining properties were affected by the side-chain structure of the dyes. These data indicate that the fluorescent dye ZMB793 could be used for the SP cell analysis. |
著作権等: | © 2012 Elsevier Ltd. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/166850 |
DOI(出版社版): | 10.1016/j.biomaterials.2012.10.059 |
PubMed ID: | 23146431 |
出現コレクション: | 学術雑誌掲載論文等 |
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