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Title: Selective development of myogenic mesenchymal cells from human embryonic and induced pluripotent stem cells.
Authors: Awaya, Tomonari  kyouindb  KAKEN_id  orcid (unconfirmed)
Kato, Takeo
Mizuno, Yuta
Chang, Hsi
Niwa, Akira  kyouindb  KAKEN_id
Umeda, Katsutsugu  kyouindb  KAKEN_id
Nakahata, Tatsutoshi  kyouindb  KAKEN_id
Heike, Toshio  kyouindb  KAKEN_id
Author's alias: 粟屋, 智就
Issue Date: 7-Dec-2012
Publisher: Public Library of Science
Journal title: PloS one
Volume: 7
Issue: 12
Thesis number: e51638
Abstract: Human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells are promising sources for the cell therapy of muscle diseases and can serve as powerful experimental tools for skeletal muscle research, provided an effective method to induce skeletal muscle cells is established. However, the current methods for myogenic differentiation from human ES cells are still inefficient for clinical use, while myogenic differentiation from human iPS cells remains to be accomplished. Here, we aimed to establish a practical differentiation method to induce skeletal myogenesis from both human ES and iPS cells. To accomplish this goal, we developed a novel stepwise culture method for the selective expansion of mesenchymal cells from cell aggregations called embryoid bodies. These mesenchymal cells, which were obtained by dissociation and re-cultivation of embryoid bodies, uniformly expressed CD56 and the mesenchymal markers CD73, CD105, CD166, and CD29, and finally differentiated into mature myotubes in vitro. Furthermore, these myogenic mesenchymal cells exhibited stable long-term engraftment in injured muscles of immunodeficient mice in vivo and were reactivated upon subsequent muscle damage, increasing in number to reconstruct damaged muscles. Our simple differentiation system facilitates further utilization of ES and iPS cells in both developmental and pathological muscle research and in serving as a practical donor source for cell therapy of muscle diseases.
Rights: © 2012 Awaya et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
DOI(Published Version): 10.1371/journal.pone.0051638
PubMed ID: 23236522
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