ダウンロード数: 331
このアイテムのファイル:
ファイル | 記述 | サイズ | フォーマット | |
---|---|---|---|---|
nar_gks897.pdf | 1.7 MB | Adobe PDF | 見る/開く |
完全メタデータレコード
DCフィールド | 値 | 言語 |
---|---|---|
dc.contributor.author | Han, Yong-Woon | en |
dc.contributor.author | Matsumoto, Tomoko | en |
dc.contributor.author | Yokota, Hiroaki | en |
dc.contributor.author | Kashiwazaki, Gengo | en |
dc.contributor.author | Morinaga, Hironobu | en |
dc.contributor.author | Hashiya, Kaori | en |
dc.contributor.author | Bando, Toshikazu | en |
dc.contributor.author | Harada, Yoshie | en |
dc.contributor.author | Sugiyama, Hiroshi | en |
dc.contributor.alternative | 韓, 龍雲 | ja |
dc.date.accessioned | 2013-01-09T04:18:39Z | - |
dc.date.available | 2013-01-09T04:18:39Z | - |
dc.date.issued | 2012-10-04 | - |
dc.identifier.issn | 0305-1048 | - |
dc.identifier.uri | http://hdl.handle.net/2433/167748 | - |
dc.description.abstract | N-methylpyrrole (Py)-N-methylimidazole (Im) polyamides are small organic molecules that bind to DNA with sequence specificity and can be used as synthetic DNA-binding ligands. In this study, five hairpin eight-ring Py-Im polyamides 1-5 with different number of Im rings were synthesized, and their binding behaviour was investigated with surface plasmon resonance assay. It was found that association rate (k(a)) of the Py-Im polyamides with their target DNA decreased with the number of Im in the Py-Im polyamides. The structures of four-ring Py-Im polyamides derived from density functional theory revealed that the dihedral angle of the Py amide carbonyl is 14∼18°, whereas that of the Im is significantly smaller. As the minor groove of DNA has a helical structure, planar Py-Im polyamides need to change their conformation to fit it upon binding to the minor groove. The data explain that an increase in planarity of Py-Im polyamide induced by the incorporation of Im reduces the association rate of Py-Im polyamides. This fundamental knowledge of the binding of Py-Im polyamides to DNA will facilitate the design of hairpin Py-Im polyamides as synthetic DNA-binding modules. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Oxford University Press | en |
dc.rights | © The Author(s) 2012. Published by Oxford University Press. | en |
dc.rights | This is an Open Access article distributed under the terms of the Creative Commons Attribution License. | en |
dc.title | Binding of hairpin pyrrole and imidazole polyamides to DNA: relationship between torsion angle and association rate constants. | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.ncid | AA00760269 | - |
dc.identifier.jtitle | Nucleic acids research | en |
dc.identifier.volume | 40 | - |
dc.identifier.issue | 22 | - |
dc.identifier.spage | 11510 | - |
dc.identifier.epage | 11517 | - |
dc.relation.doi | 10.1093/nar/gks897 | - |
dc.textversion | publisher | - |
dc.identifier.pmid | 23042247 | - |
dcterms.accessRights | open access | - |
出現コレクション: | 学術雑誌掲載論文等 |
このリポジトリに保管されているアイテムはすべて著作権により保護されています。