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タイトル: | Photoreactivities of 5-Bromouracil-containing RNAs. |
著者: | Morinaga, Hironobu Kizaki, Seiichiro Takenaka, Tomohiro Kanesato, Shuhei Sannohe, Yuta Tashiro, Ryu Sugiyama, Hiroshi https://orcid.org/0000-0001-8923-5946 (unconfirmed) |
著者名の別形: | 杉山, 弘 |
キーワード: | 5-Bromouracil Electron transfer Photoreaction RNA |
発行日: | 15-Jan-2013 |
出版者: | Elsevier Ltd. |
誌名: | Bioorganic & medicinal chemistry |
巻: | 21 |
号: | 2 |
開始ページ: | 466 |
終了ページ: | 469 |
抄録: | 5-Bromouracil ((Br)U) was incorporated into three types of synthetic RNA and the products of the photoirradiated (Br)U-containing RNAs were investigated using HPLC and MS analysis. The photoirradiation of r(GCA(Br)UGC)(2) and r(CGAA(Br)UUGC)/r(GCAAUUCG) in A-form RNA produced the corresponding 2'-keto adenosine ((keto)A) product at the 5'-neighboring nucleotide, such as r(GC(keto)AUGC) and r(CGA(keto)AUUGC), respectively. The photoirradiation of r(CGCG(Br)UGCG)/r(C(m)GCAC(m)GCG) in Z-form RNA produced the 2'-keto guanosine ((keto)G) product r(CGC(keto)GUGCG), whereas almost no products were observed from the photoirradiation of r(CGCG(Br)UGCG)/r(C(m)GCAC(m)GCG) in A-form RNA. The present results indicate clearly that hydrogen (H) abstraction by the photochemically generated uracil-5-yl radical selectively occurs at the C2' position to provide a 2'-keto RNA product. |
著作権等: | © 2012 Elsevier Ltd. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/168517 |
DOI(出版社版): | 10.1016/j.bmc.2012.11.010 |
PubMed ID: | 23266180 |
出現コレクション: | 学術雑誌掲載論文等 |
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