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dc.contributor.author | Jo, Jun-Ichiro | en |
dc.contributor.author | Lin, Xue | en |
dc.contributor.author | Nakahara, Teppei | en |
dc.contributor.author | Aoki, Ichio | en |
dc.contributor.author | Saga, Tsuneo | en |
dc.contributor.author | Tabata, Yasuhiko | en |
dc.contributor.alternative | 田畑, 泰彦 | ja |
dc.date.accessioned | 2013-01-18T04:53:04Z | - |
dc.date.available | 2013-01-18T04:53:04Z | - |
dc.date.issued | 2012-12 | - |
dc.identifier.issn | 1937-3341 | - |
dc.identifier.uri | http://hdl.handle.net/2433/168521 | - |
dc.description.abstract | The objective of this study was to design and prepare a new contrast agent of magnetic resonance (MR) imaging for the evaluation of therapeutic angiogenesis. Diethylenetriaminepentaacetic acid (DTPA) residue of a chelator was chemically introduced to dextran with a molecular weight of 74,000 (dextran-DTPA). Cyclic peptide containing an arginine-glycine-aspartic acid (RGD) sequence (cyclic RGD) with an inherent affinity for the α(v)β(3) integrin was then introduced to dextran-DTPA (Cyclic RGD-dextran-DTPA). Gd(3+) was added to cyclic RGD-dextran-DTPA to prepare a dextran-based MR contrast agent (Cyclic RGD-dextran-DTPA-Gd). Cyclic RGD-dextran-DTPA-Gd had affinity for cells expressing the α(v)β(3) integrin and showed a higher longitudinal relaxivity compared with DTPA-Gd of an MR contrast agent clinically used. Right femoral, external iliac, and deep femoral and circumflex arteries and veins were surgically ligated to prepare a mouse model of hindlimb ischemia. A laser Doppler analysis and histological evaluation confirmed that hindlimb ischemia healed naturally and was accompanied by angiogenesis, while α(v)β(3) integrin was expressed in the ischemic-angiogenic region without any treatment. Mice at 7 days after vascular ligation were used as an angiogenesis model. When intravenously injected into mice with hindlimb ischemia, cyclic RGD-dextran-DTPA accumulated in the ischemic-angiogenic region and showed the MR ability to detect the ischemic-angiogenic region. It is concluded that cyclic RGD-dextran-DTPA-Gd is a promising material for evaluation of therapeutic angiogenesis. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Mary Ann Liebert, Inc. | en |
dc.rights | This is a copy of an article published in the Tissue Engineering Part A. | en |
dc.rights | ©2012 Mary Ann Liebert, Inc. | en |
dc.rights | "Tissue Engineering Part A" is available online at: http://online.liebertpub.com. | en |
dc.title | Preparation of polymer-based magnetic resonance imaging contrast agent to visualize therapeutic angiogenesis. | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.ncid | AA12306003 | - |
dc.identifier.jtitle | Tissue engineering. Part A | en |
dc.identifier.volume | 19 | - |
dc.identifier.issue | 1-2 | - |
dc.identifier.spage | 30 | - |
dc.identifier.epage | 39 | - |
dc.relation.doi | 10.1089/ten.TEA.2012.0131 | - |
dc.textversion | publisher | - |
dc.identifier.pmid | 22838390 | - |
dcterms.accessRights | open access | - |
出現コレクション: | 学術雑誌掲載論文等 |
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