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dc.contributor.author | Nishikimi, Toshio | en |
dc.contributor.author | Okamoto, Hiroyuki | en |
dc.contributor.author | Nakamura, Masahiro | en |
dc.contributor.author | Ogawa, Naoko | en |
dc.contributor.author | Horii, Kazukiyo | en |
dc.contributor.author | Nagata, Kiyoshi | en |
dc.contributor.author | Nakagawa, Yasuaki | en |
dc.contributor.author | Kinoshita, Hideyuki | en |
dc.contributor.author | Yamada, Chinatsu | en |
dc.contributor.author | Nakao, Kazuhiro | en |
dc.contributor.author | Minami, Takeya | en |
dc.contributor.author | Kuwabara, Yoshihiro | en |
dc.contributor.author | Kuwahara, Koichiro | en |
dc.contributor.author | Masuda, Izuru | en |
dc.contributor.author | Kangawa, Kenji | en |
dc.contributor.author | Minamino, Naoto | en |
dc.contributor.author | Nakao, Kazuwa | en |
dc.contributor.alternative | 錦見, 俊雄 | ja |
dc.date.accessioned | 2013-02-18T06:36:07Z | - |
dc.date.available | 2013-02-18T06:36:07Z | - |
dc.date.issued | 2013-01-24 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | http://hdl.handle.net/2433/169792 | - |
dc.description.abstract | [Background]Recent studies have shown that in addition to brain (or B-type) natriuretic peptide (BNP) and the N-terminal proBNP fragment, levels of intact proBNP are also increased in heart failure. Moreover, present BNP immunoassays also measure proBNP, as the anti-BNP antibody cross-reacts with proBNP. It is important to know the exact levels of proBNP in heart failure, because elevation of the low-activity proBNP may be associated with the development of heart failure. [Methodology/Principal Findings]We therefore established a two-step immunochemiluminescent assay for total BNP (BNP+proBNP) and proBNP using monoclonal antibodies and glycosylated proBNP as a standard. The assay enables measurement of plasma total BNP and proBNP within only 7 h, without prior extraction of the plasma. The detection limit was 0.4 pmol/L for a 50-µl plasma sample. Within-run CVs ranged from 5.2%–8.0% in proBNP assay and from 7.0%–8.4% in total BNP assay, and between-run CVs ranged from 5.3–7.4% in proBNP assay and from 2.9%–9.5% in total BNP assay, respectively. The dilution curves for plasma samples showed good linearity (correlation coefficients = 0.998–1.00), and analytical recovery was 90–101%. The mean total BNP and proBNP in plasma from 116 healthy subjects were 1.4±1.2 pM and 1.0±0.7 pM, respectively, and were 80±129 pM and 42±70 pM in 32 heart failure patients. Plasma proBNP levels significantly correlate with age in normal subjects. [Conclusions/Significance]Our immunochemiluminescent assay is sufficiently rapid and precise for routine determination of total BNP and proBNP in human plasma. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Public Library of Science | en |
dc.rights | © 2013 Nishikimi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | en |
dc.title | Direct Immunochemiluminescent Assay for proBNP and Total BNP in Human Plasma proBNP and Total BNP Levels in Normal and Heart Failure. | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | PloS one | en |
dc.identifier.volume | 8 | - |
dc.identifier.issue | 1 | - |
dc.relation.doi | 10.1371/journal.pone.0053233 | - |
dc.textversion | publisher | - |
dc.identifier.artnum | e53233 | - |
dc.identifier.pmid | 23365636 | - |
dcterms.accessRights | open access | - |
出現コレクション: | 学術雑誌掲載論文等 |
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