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タイトル: | Enzymatic characterization of human immunodeficiency virus type 1 reverse transcriptase for use in cDNA synthesis. |
著者: | Konishi, Atsushi Shinomura, Mayu Yasukawa, Kiyoshi |
著者名の別形: | 保川, 清 |
キーワード: | cDNA Formamide HIV-1 MMLV Reverse transcriptase |
発行日: | Jan-2013 |
出版者: | Springer-Verlag |
誌名: | Applied biochemistry and biotechnology |
巻: | 169 |
号: | 1 |
開始ページ: | 77 |
終了ページ: | 87 |
抄録: | The aim of this study is to explore the advantages of using human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) in cDNA synthesis. Recombinant HIV-1 group M (HIV-1 M) RT and HIV-1 group O (HIV-1 O) RT were produced in an Escherichia coli expression system. In the incorporation of dTTP into poly(rA)-p(dT)(15) (T/P), the K (m) values for dTTP of HIV-1 M RT and HIV-1 O RT were 8 and 12 % of that of Moloney murine leukemia virus (MMLV) RT, respectively, and the K (m) values for T/P were 25 and 23 % of that of MMLV RT, respectively. Compared with MMLV RT, HIV-1 M RT and HIV-1 O RT were less susceptible to formamide, which is frequently used for cDNA synthesis with a G + C-rich RNA to improve specificity. The high substrate affinity and low susceptibility to formamide of HIV-1 RT might be advantageous for its use in cDNA synthesis. |
著作権等: | The final publication is available at www.springerlink.com This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 |
URI: | http://hdl.handle.net/2433/169949 |
DOI(出版社版): | 10.1007/s12010-012-9953-8 |
PubMed ID: | 23149716 |
出現コレクション: | 学術雑誌掲載論文等 |
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