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Title: Enzymatic characterization of human immunodeficiency virus type 1 reverse transcriptase for use in cDNA synthesis.
Authors: Konishi, Atsushi
Shinomura, Mayu
Yasukawa, Kiyoshi  kyouindb  KAKEN_id
Author's alias: 保川, 清
Keywords: cDNA
Formamide
HIV-1
MMLV
Reverse transcriptase
Issue Date: Jan-2013
Publisher: Springer-Verlag
Journal title: Applied biochemistry and biotechnology
Volume: 169
Issue: 1
Start page: 77
End page: 87
Abstract: The aim of this study is to explore the advantages of using human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) in cDNA synthesis. Recombinant HIV-1 group M (HIV-1 M) RT and HIV-1 group O (HIV-1 O) RT were produced in an Escherichia coli expression system. In the incorporation of dTTP into poly(rA)-p(dT)(15) (T/P), the K (m) values for dTTP of HIV-1 M RT and HIV-1 O RT were 8 and 12 % of that of Moloney murine leukemia virus (MMLV) RT, respectively, and the K (m) values for T/P were 25 and 23 % of that of MMLV RT, respectively. Compared with MMLV RT, HIV-1 M RT and HIV-1 O RT were less susceptible to formamide, which is frequently used for cDNA synthesis with a G + C-rich RNA to improve specificity. The high substrate affinity and low susceptibility to formamide of HIV-1 RT might be advantageous for its use in cDNA synthesis.
Rights: The final publication is available at www.springerlink.com
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/169949
DOI(Published Version): 10.1007/s12010-012-9953-8
PubMed ID: 23149716
Appears in Collections:Journal Articles

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