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Title: Lymphocyte-stromal cell interaction induces IL-7 expression by interferon regulatory factors.
Authors: Sekai, Miho  kyouindb  KAKEN_id
Tani-Ichi, Shizue
Yoneyama, Mitsutoshi
Fujita, Takashi  kyouindb  KAKEN_id
Kina, Tatsuo
Ikuta, Koichi  kyouindb  KAKEN_id
Author's alias: 生田, 宏一
Keywords: Cytokine
Cell adhesion
Transcription factor
Stromal cell
Issue Date: Jan-2013
Publisher: Elsevier Ltd.
Journal title: Molecular immunology
Volume: 54
Issue: 3-4
Start page: 378
End page: 385
Abstract: The interaction between lymphocytes and stromal cells plays important roles in coordinated development of early lymphocytes. IL-7 is an essential cytokine for early lymphocyte development produced by stromal cells in the thymus and bone marrow. Although IL-7 is induced by interaction of early lymphocytes and stromal cells, its molecular basis is still unknown. To address this question, we employed co-culture system with an IL-7-dependent pre-B cell line, DW34, and a thymic stromal cell line, TSt-4. Co-culture with DW34 cells enhanced the levels of IL-7 transcripts in TSt-4 cells. Interestingly, the co-culture also induced transcripts of IFN-α and IFN-β but not of IFN-γ. In addition, exogenous IFN-β stimulation increased the levels of IL-7 transcripts in TSt-4 cells. Next, to elucidate the molecular mechanism of IL-7 induction, we analyzed the IL-7 promoter activity by reporter assay. The IL-7 promoter showed specific transcriptional activity in TSt-4 cells. An interferon-stimulated response element (ISRE) in the IL-7 promoter was essential for the induction of IL-7 transcription by both co-culture and IFN-β stimulation. Finally, overexpression of wild-type and dominant-negative forms of interferon regulatory factors (IRFs) activated and repressed, respectively, the IL-7 promoter in TSt-4 cells. Collectively, these results suggested that IRFs activated by lymphocyte adhesion induce IL-7 transcription through ISRE in stromal cells and that type I IFNs may be involved in the activation of IRFs. Thus, this study implied a physiological function of the IFN/IRF signal during lymphocyte development.
Rights: © 2013 Published by Elsevier Ltd.
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
DOI(Published Version): 10.1016/j.molimm.2013.01.002
PubMed ID: 23376291
Appears in Collections:Journal Articles

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