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dc.contributor.author | Mizota, Toshiyuki | en |
dc.contributor.author | Tsujikawa, Hiroshi | en |
dc.contributor.author | Shoda, Takehiro | en |
dc.contributor.author | Fukuda, Kazuhiko | en |
dc.contributor.alternative | 溝田, 敏幸 | ja |
dc.date.accessioned | 2013-03-19T06:53:48Z | - |
dc.date.available | 2013-03-19T06:53:48Z | - |
dc.date.issued | 2013-02 | - |
dc.identifier.issn | 0913-8668 | - |
dc.identifier.uri | http://hdl.handle.net/2433/172077 | - |
dc.description.abstract | [Purpose]In this study, we aimed to investigate the effect of morphine on the activation of extracellular signal-regulated kinase (ERK) and nuclear factor-κB (NF-κB), both of which play crucial roles in T-cell activation. [Methods]Human CD3+ T cells and Jurkat T cells were stimulated by anti-CD3 antibody or phorbol 12-myristate 13-acetate plus ionomycin with or without 24-h pretreatment with morphine. Activation of ERK was assessed by immunoblot analysis of phosphorylated ERK. Activation of the NF-κB signaling pathway was examined by analyzing nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (IκBα) phosphorylation using immunoblotting, and interleukin-2 (IL-2) gene expression using quantitative real-time reverse-transcriptase polymerase chain reaction. [Results]Morphine pretreatment enhanced ERK phosphorylation, but inhibited IκBα phosphorylation and IL-2 gene expression in activated T cells. The effects of morphine on ERK phosphorylation and IL-2 gene expression were not antagonized by naloxone. We detected κ-opioid receptor transcript in T cells, but U50, 488, a κ-receptor-selective agonist, did not enhance ERK phosphorylation. [Conclusion]Morphine enhances ERK signaling, whereas it inhibits NF-κB signaling in activated human T cells. These effects of morphine are unlikely to be mediated by known opioid receptors. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Springer Japan | en |
dc.rights | The final publication is available at www.springerlink.com | en |
dc.rights | This is not the published version. Please cite only the published version. | en |
dc.rights | この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 | ja |
dc.subject | Morphine | en |
dc.subject | Erk1/2 | en |
dc.subject | Nuclear factor-κB | en |
dc.subject | Opioid-induced immunomodulation | en |
dc.title | Dual modulation of the T-cell receptor-activated signal transduction pathway by morphine in human T lymphocytes. | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.ncid | AA10852931 | - |
dc.identifier.jtitle | Journal of anesthesia | en |
dc.identifier.volume | 27 | - |
dc.identifier.issue | 1 | - |
dc.identifier.spage | 80 | - |
dc.identifier.epage | 87 | - |
dc.relation.doi | 10.1007/s00540-012-1472-9 | - |
dc.textversion | author | - |
dc.identifier.pmid | 22932814 | - |
dcterms.accessRights | open access | - |
出現コレクション: | 学術雑誌掲載論文等 |
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