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Title: Histone chaperone activity of Fanconi anemia proteins, FANCD2 and FANCI, is required for DNA crosslink repair.
Authors: Sato, Koichi
Ishiai, Masamichi  KAKEN_id
Toda, Kazue
Furukoshi, Satoshi
Osakabe, Akihisa
Tachiwana, Hiroaki
Takizawa, Yoshimasa
Kagawa, Wataru
Kitao, Hiroyuki
Dohmae, Naoshi
Obuse, Chikashi
Kimura, Hiroshi
Takata, Minoru  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-4926-3675 (unconfirmed)
Kurumizaka, Hitoshi
Author's alias: 高田, 穣
Keywords: DNA repair
FANCD2
FANCI
Fanconi anaemia
histone chaperone
Issue Date: 29-Aug-2012
Publisher: Nature Publishing Group
Journal title: The EMBO journal
Volume: 31
Issue: 17
Start page: 3524
End page: 3536
Abstract: Fanconi anaemia (FA) is a rare hereditary disorder characterized by genomic instability and cancer susceptibility. A key FA protein, FANCD2, is targeted to chromatin with its partner, FANCI, and plays a critical role in DNA crosslink repair. However, the molecular function of chromatin-bound FANCD2-FANCI is still poorly understood. In the present study, we found that FANCD2 possesses nucleosome-assembly activity in vitro. The mobility of histone H3 was reduced in FANCD2-knockdown cells following treatment with an interstrand DNA crosslinker, mitomycin C. Furthermore, cells harbouring FANCD2 mutations that were defective in nucleosome assembly displayed impaired survival upon cisplatin treatment. Although FANCI by itself lacked nucleosome-assembly activity, it significantly stimulated FANCD2-mediated nucleosome assembly. These observations suggest that FANCD2-FANCI may regulate chromatin dynamics during DNA repair.
Rights: © 2012 European Molecular Biology Organization.
This is not the published version. Please cite only the published version.
この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/172223
DOI(Published Version): 10.1038/emboj.2012.197
PubMed ID: 22828868
Appears in Collections:Journal Articles

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