Downloads: 388
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
emboj.2012.197.pdf | 1.91 MB | Adobe PDF | View/Open |
Title: | Histone chaperone activity of Fanconi anemia proteins, FANCD2 and FANCI, is required for DNA crosslink repair. |
Authors: | Sato, Koichi Ishiai, Masamichi ![]() Toda, Kazue Furukoshi, Satoshi Osakabe, Akihisa Tachiwana, Hiroaki Takizawa, Yoshimasa Kagawa, Wataru Kitao, Hiroyuki Dohmae, Naoshi Obuse, Chikashi Kimura, Hiroshi Takata, Minoru ![]() ![]() ![]() Kurumizaka, Hitoshi |
Author's alias: | 高田, 穣 |
Keywords: | DNA repair FANCD2 FANCI Fanconi anaemia histone chaperone |
Issue Date: | 29-Aug-2012 |
Publisher: | Nature Publishing Group |
Journal title: | The EMBO journal |
Volume: | 31 |
Issue: | 17 |
Start page: | 3524 |
End page: | 3536 |
Abstract: | Fanconi anaemia (FA) is a rare hereditary disorder characterized by genomic instability and cancer susceptibility. A key FA protein, FANCD2, is targeted to chromatin with its partner, FANCI, and plays a critical role in DNA crosslink repair. However, the molecular function of chromatin-bound FANCD2-FANCI is still poorly understood. In the present study, we found that FANCD2 possesses nucleosome-assembly activity in vitro. The mobility of histone H3 was reduced in FANCD2-knockdown cells following treatment with an interstrand DNA crosslinker, mitomycin C. Furthermore, cells harbouring FANCD2 mutations that were defective in nucleosome assembly displayed impaired survival upon cisplatin treatment. Although FANCI by itself lacked nucleosome-assembly activity, it significantly stimulated FANCD2-mediated nucleosome assembly. These observations suggest that FANCD2-FANCI may regulate chromatin dynamics during DNA repair. |
Rights: | © 2012 European Molecular Biology Organization. This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 |
URI: | http://hdl.handle.net/2433/172223 |
DOI(Published Version): | 10.1038/emboj.2012.197 |
PubMed ID: | 22828868 |
Appears in Collections: | Journal Articles |

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.