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dc.contributor.author | Nieh, Chi-Hua | en |
dc.contributor.author | Tsujimura, Seiya | en |
dc.contributor.author | Shirai, Osamu | en |
dc.contributor.author | Kano, Kenji | en |
dc.contributor.alternative | 加納, 健司 | ja |
dc.date.accessioned | 2013-04-04T00:49:57Z | - |
dc.date.available | 2013-04-04T00:49:57Z | - |
dc.date.issued | 2013-03-12 | - |
dc.identifier.issn | 0003-2670 | - |
dc.identifier.uri | http://hdl.handle.net/2433/173085 | - |
dc.description.abstract | Pentacyanoferrate-bound poly(1-vinylimidazole) (PVI[Fe(CN)5]) was selected as a mediator for amperometric creatinine determination based on the reductive H2O2 detection. Creatinine amidohydrolase (CNH), creatine amidohydrolase (CRH), sarcosine oxidase (SOD), peroxidase (POD), and PVI[Fe(CN)5] were crosslinked with poly(ethylene glycol) diglycidyl ether (PEGDGE) on a glassy carbon (GC) electrode for a creatinine biosensor fabrication. Reduction current was monitored at −0.1 V in the presence of creatinine and O2. It is revealed that PVI[Fe(CN)5] is suitable as a mediator for a bioelectrocatalytic reaction of POD, since PVI[Fe(CN)5] neither reacts with reactants nor works as an electron acceptor of SOD. The amounts of PVI[Fe(CN)5], PEGDGE, and enzymes were optimized toward creatinine detection. Nafion as a protecting film successfully prevented the enzyme layer from interferences. The detection limit and linear range in creatinine determination were 12 μM and 12–500 μM (R[2]= 0.993), respectively, and the sensitivity was 11 mA cm[−2] M[−1], which is applicable for urine creatinine tests. The results of the creatinine determination for four urine samples measured with this proposed method were compared with Jaffe method, and a good correlation was obtained between the results. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier B.V. | en |
dc.rights | © 2013 Elsevier B.V. | en |
dc.rights | この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 | ja |
dc.rights | This is not the published version. Please cite only the published version. | en |
dc.subject | Pentacyanoferrate-bound poly(1-vinylimidazole) | en |
dc.subject | Creatinine amidohydrolase | en |
dc.subject | Reductive H2O2 detection | en |
dc.subject | Peroxidase | en |
dc.subject | Nafion | en |
dc.subject | Urine creatinine test | en |
dc.title | Amperometric biosensor based on reductive H2O2 detection using pentacyanoferrate-bound polymer for creatinine determination. | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.ncid | AA00524834 | - |
dc.identifier.jtitle | Analytica chimica acta | en |
dc.identifier.volume | 767 | - |
dc.identifier.spage | 128 | - |
dc.identifier.epage | 133 | - |
dc.relation.doi | 10.1016/j.aca.2012.12.052 | - |
dc.textversion | author | - |
dc.identifier.pmid | 23452796 | - |
dcterms.accessRights | open access | - |
出現コレクション: | 学術雑誌掲載論文等 |
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