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dc.contributor.author | Masunaga, Shin-Ichiro | en |
dc.contributor.author | Sakurai, Yoshinori | en |
dc.contributor.author | Tanaka, Hiroki | en |
dc.contributor.author | Suzuki, Minoru | en |
dc.contributor.author | Kondo, Natsuko | en |
dc.contributor.author | Narabayashi, Masaru | en |
dc.contributor.author | Maruhashi, Akira | en |
dc.contributor.author | Ono, Koji | en |
dc.contributor.alternative | 増永, 慎一郎 | ja |
dc.date.accessioned | 2013-04-04T01:53:59Z | - |
dc.date.available | 2013-04-04T01:53:59Z | - |
dc.date.issued | 2013-03 | - |
dc.identifier.issn | 0449-3060 | - |
dc.identifier.uri | http://hdl.handle.net/2433/173088 | - |
dc.description.abstract | Labeling of proliferating (P) cells in mice bearing EL4 tumors was achieved by continuous administration of 5-bromo-2'-deoxyuridine (BrdU). Tumors were irradiated with γ-rays at 1 h after pimonidazole administration followed by caffeine or wortmannin treatment. Twenty-four hours later, assessment of the responses of quiescent (Q) and total (= P + Q) cell populations were based on the frequencies of micronucleation and apoptosis using immunofluorescence staining for BrdU. The response of the pimonidazole-unlabeled tumor cell fractions was assessed by means of apoptosis frequency using immunofluorescence staining for pimonidazole. The pimonidazole-unlabeled cell fraction showed significantly enhanced radio-sensitivity compared with the whole cell fraction more remarkably in Q cells than total cells. However, a significantly greater decrease in radio-sensitivity in the pimonidazole-unlabeled than the whole cell fraction, evaluated using an assay performed 24 hours after irradiation, was more clearly observed in Q cells than total cells. In both the pimonidazole-unlabeled and the whole cell fractions, wortmannin efficiently suppressed the reduction in sensitivity due to delayed assay. Wortmannin combined with γ-ray irradiation is useful for suppressing the recovery from radiation-induced damage especially in the pimonidazole-unlabeled cell fraction within the total and Q tumor cell populations. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Oxford University Press | en |
dc.rights | © The Author 2012. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited | en |
dc.subject | Quiescent cell | en |
dc.subject | recovery from radiation-induced damage | en |
dc.subject | pimonidazole | en |
dc.subject | wortmannin | en |
dc.subject | caffeine | en |
dc.title | Wortmannin efficiently suppresses the recovery from radiation-induced damage in pimonidazole-unlabeled quiescent tumor cell population. | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.ncid | AA00705792 | - |
dc.identifier.jtitle | Journal of radiation research | en |
dc.identifier.volume | 54 | - |
dc.identifier.issue | 2 | - |
dc.identifier.spage | 221 | - |
dc.identifier.epage | 229 | - |
dc.relation.doi | 10.1093/jrr/rrs094 | - |
dc.textversion | publisher | - |
dc.identifier.pmid | 23097299 | - |
dcterms.accessRights | open access | - |
出現コレクション: | 学術雑誌掲載論文等 |
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