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ファイル | 記述 | サイズ | フォーマット | |
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j.febslet.2013.02.003.pdf | 813.54 kB | Adobe PDF | 見る/開く |
タイトル: | A fluorescence resonance energy transfer (FRET)-based redox sensor reveals physiological role of thioredoxin in the yeast Saccharomyces cerevisiae. |
著者: | Oku, Masahide https://orcid.org/0000-0001-5991-6281 (unconfirmed) Hoseki, Jun Ichiki, Yayoi Sakai, Yasuyoshi |
著者名の別形: | 阪井, 康能 |
キーワード: | FRET Redox regulation Thioredoxin Yeast physiology |
発行日: | 18-Mar-2013 |
出版者: | Elsevier B.V. |
誌名: | FEBS letters |
巻: | 587 |
号: | 6 |
開始ページ: | 793 |
終了ページ: | 798 |
抄録: | The physiological roles of the thioredoxin isozymes in the yeast Saccharomyces cerevisiae were investigated using a novel FRET-based redox probe, Redoxfluor. After establishing responsiveness of the probe toward thioredoxin, we followed the fluorescence signal of Redoxfluor expressed in the yeast and found that one of the thioredoxin isozymes, Trx2, was required for maintaining the redox status when stationary culture of the organism was exposed to starvation and mild-heat stresses. The failure to maintain redox balance under the tested condition preceded decreased viability of the trx2 mutants, indicating the functional importance of the cytoplasmic thioredoxin in adaptation to environmental changes. |
著作権等: | © 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 |
URI: | http://hdl.handle.net/2433/173130 |
DOI(出版社版): | 10.1016/j.febslet.2013.02.003 |
PubMed ID: | 23416294 |
出現コレクション: | 学術雑誌掲載論文等 |
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