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Title: Bile canalicular abnormalities in the early phase of a mouse model of sclerosing cholangitis.
Authors: Miyao, Masashi  kyouindb  KAKEN_id
Ozeki, Munetaka  kyouindb  KAKEN_id
Abiru, Hitoshi
Manabe, Sho
Kotani, Hirokazu  kyouindb  KAKEN_id
Tsuruyama, Tatsuaki  kyouindb  KAKEN_id
Tamaki, Keiji  kyouindb  KAKEN_id
Author's alias: 宮尾, 昌
玉木, 敬二
Keywords: Bile canaliculus
3, 5-Diethoxycarbonyl-1, 4-dihydrocollidine
Ductular reactions
Issue Date: Mar-2013
Publisher: Elsevier Ltd.
Journal title: Digestive and liver disease
Volume: 45
Issue: 3
Start page: 216
End page: 225
Abstract: [Background]The bile canaliculus is the smallest and first biliary channel and is formed by two or three adjacent hepatocytes. Previous studies of chronic cholangiopathies such as primary sclerosing cholangitis have focused on the bile ductules. However, little is known about the pathological alterations in bile canaliculi in the early phase of cholangiopathies. [Aim]To characterize the bile canalicular morphology in the early phase of sclerosing cholangitis we used 3, 5-diethoxycarbonyl-1, 4-dihydrocollidine-induced mouse model of sclerosing cholangitis. [Methods]Mice were fed a diet with 3, 5-diethoxycarbonyl-1, 4-dihydrocollidine (0.1%). Serum biochemical, histological, immunohistochemical, and electron microscopic analyses were performed 1, 2, 4, and 7 days after feeding. [Results]All experimental groups showed significantly increased serum aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase levels. From day 1, bile canalicular abnormalities such as dilatation and meandering and loss of microvilli were observed. After bile canalicular abnormalities had appeared, substantial infiltration of inflammatory cells was observed amongst the necrotic cells and periductal region. After these inflammatory changes, cholangiocytes proliferated in the portal area and formed ductular reactions. Finally, periductal fibrosis appeared. [Conclusion]This study provides novel evidence of the occurrence of bile canalicular abnormalities during the early phase of sclerosing cholangitis.
Rights: © 2012 Editrice Gastroenterologica Italiana S.r.l. Published by Elsevier Ltd.
この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。This is not the published version. Please cite only the published version.
DOI(Published Version): 10.1016/j.dld.2012.09.007
PubMed ID: 23107486
Appears in Collections:Journal Articles

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