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Title: Robust and Highly-Efficient Differentiation of Functional Monocytic Cells from Human Pluripotent Stem Cells under Serum- and Feeder Cell-Free Conditions.
Authors: Yanagimachi, Masakatsu D
Niwa, Akira  kyouindb  KAKEN_id
Tanaka, Takayuki
Honda-Ozaki, Fumiko
Nishimoto, Seiko
Murata, Yuuki
Yasumi, Takahiro  kyouindb  KAKEN_id
Ito, Jun
Tomida, Shota
Oshima, Koichi
Asaka, Isao  kyouindb  KAKEN_id
Goto, Hiroaki
Heike, Toshio  kyouindb  KAKEN_id
Nakahata, Tatsutoshi  kyouindb  KAKEN_id
Saito, Megumu K
Author's alias: 齋藤, 潤
Issue Date: 3-Apr-2013
Publisher: Public Library of Science
Journal title: PloS one
Volume: 8
Issue: 4
Thesis number: e59243
Abstract: Monocytic lineage cells (monocytes, macrophages and dendritic cells) play important roles in immune responses and are involved in various pathological conditions. The development of monocytic cells from human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) is of particular interest because it provides an unlimited cell source for clinical application and basic research on disease pathology. Although the methods for monocytic cell differentiation from ESCs/iPSCs using embryonic body or feeder co-culture systems have already been established, these methods depend on the use of xenogeneic materials and, therefore, have a relatively poor-reproducibility. Here, we established a robust and highly-efficient method to differentiate functional monocytic cells from ESCs/iPSCs under serum- and feeder cell-free conditions. This method produced 1.3×10(6)±0.3×10(6) floating monocytes from approximately 30 clusters of ESCs/iPSCs 5-6 times per course of differentiation. Such monocytes could be differentiated into functional macrophages and dendritic cells. This method should be useful for regenerative medicine, disease-specific iPSC studies and drug discovery.
Rights: © 2013 Yanagimachi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
DOI(Published Version): 10.1371/journal.pone.0059243
PubMed ID: 23573196
Appears in Collections:Journal Articles

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