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タイトル: | Robust and Highly-Efficient Differentiation of Functional Monocytic Cells from Human Pluripotent Stem Cells under Serum- and Feeder Cell-Free Conditions. |
著者: | Yanagimachi, Masakatsu D Niwa, Akira Tanaka, Takayuki Honda-Ozaki, Fumiko Nishimoto, Seiko Murata, Yuuki Yasumi, Takahiro Ito, Jun Tomida, Shota Oshima, Koichi Asaka, Isao Goto, Hiroaki Heike, Toshio Nakahata, Tatsutoshi Saito, Megumu K |
著者名の別形: | 齋藤, 潤 |
発行日: | 3-Apr-2013 |
出版者: | Public Library of Science |
誌名: | PloS one |
巻: | 8 |
号: | 4 |
論文番号: | e59243 |
抄録: | Monocytic lineage cells (monocytes, macrophages and dendritic cells) play important roles in immune responses and are involved in various pathological conditions. The development of monocytic cells from human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) is of particular interest because it provides an unlimited cell source for clinical application and basic research on disease pathology. Although the methods for monocytic cell differentiation from ESCs/iPSCs using embryonic body or feeder co-culture systems have already been established, these methods depend on the use of xenogeneic materials and, therefore, have a relatively poor-reproducibility. Here, we established a robust and highly-efficient method to differentiate functional monocytic cells from ESCs/iPSCs under serum- and feeder cell-free conditions. This method produced 1.3×10(6)±0.3×10(6) floating monocytes from approximately 30 clusters of ESCs/iPSCs 5-6 times per course of differentiation. Such monocytes could be differentiated into functional macrophages and dendritic cells. This method should be useful for regenerative medicine, disease-specific iPSC studies and drug discovery. |
著作権等: | © 2013 Yanagimachi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
URI: | http://hdl.handle.net/2433/173407 |
DOI(出版社版): | 10.1371/journal.pone.0059243 |
PubMed ID: | 23573196 |
出現コレクション: | 学術雑誌掲載論文等 |
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