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Title: Radiosensitivity of pimonidazole-unlabelled intratumour quiescent cell population to γ-rays, accelerated carbon ion beams and boron neutron capture reaction.
Authors: Masunaga, S  kyouindb  KAKEN_id
Sakurai, Y  kyouindb  KAKEN_id  orcid (unconfirmed)
Tanaka, H  kyouindb  KAKEN_id
Hirayama, R
Matsumoto, Y
Uzawa, A
Suzuki, M  kyouindb  KAKEN_id  orcid (unconfirmed)
Kondo, N  kyouindb  KAKEN_id
Narabayashi, M  KAKEN_id
Maruhashi, A
Ono, K
Author's alias: 増永, 慎一郎
Keywords: quiescent cell
carbon ion beam
boron neutron capture reaction
Issue Date: Jan-2013
Publisher: British Institute of Radiology
Journal title: British journal of radiology
Volume: 86
Thesis number: 20120302
Abstract: [Objectives] To detect the radiosensitivity of intratumour quiescent (Q) cells unlabelled with pimonidazole to accelerated carbon ion beams and the boron neutron capture reaction (BNCR). [Methods] EL4 tumour-bearing C57BL/J mice received 5-bromo-2′-deoxyuridine (BrdU) continuously to label all intratumour proliferating (P) cells. After the administration of pimonidazole, tumours were irradiated with γ-rays, accelerated carbon ion beams or reactor neutron beams with the prior administration of a [10]B-carrier. Responses of intratumour Q and total (P+Q) cell populations were assessed based on frequencies of micronucleation and apoptosis using immunofluorescence staining for BrdU. The response of pimonidazole-unlabelled tumour cells was assessed by means of apoptosis frequency using immunofluorescence staining for pimonidazole. [Results] Following γ-ray irradiation, the pimonidazole-unlabelled tumour cell fraction showed significantly enhanced radiosensitivity compared with the whole tumour cell fraction, more remarkably in the Q than total cell populations. However, a significantly greater decrease in radiosensitivity in the pimonidazole-unlabelled cell fraction, evaluated using a delayed assay or a decrease in radiation dose rate, was more clearly observed among the Q than total cells. These changes in radiosensitivity were suppressed following carbon ion beam and neutron beam-only irradiaton. In the BNCR, the use of a[10]B-carrier, especially L-para-boronophenylalanine-[10]B, enhanced the sensitivity of the pimonidazole-unlabelled cells more clearly in the Q than total cells. [Conclusion] The radiosensitivity of the pimonidazole-unlabelled cell fraction depends on the quality of radiation delivered and characteristics of the [10]B-carrier used in the BNCR.
Rights: © 2013 The British Institute of Radiology
This is not the published version. Please cite only the published version.
DOI(Published Version): 10.1259/bjr.20120302
PubMed ID: 23255546
Appears in Collections:Journal Articles

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