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Title: | Radiosensitivity of pimonidazole-unlabelled intratumour quiescent cell population to γ-rays, accelerated carbon ion beams and boron neutron capture reaction. |
Authors: | Masunaga, S ![]() Sakurai, Y ![]() ![]() ![]() Tanaka, H ![]() ![]() Hirayama, R Matsumoto, Y Uzawa, A Suzuki, M ![]() ![]() ![]() Kondo, N ![]() ![]() Narabayashi, M ![]() Maruhashi, A Ono, K |
Author's alias: | 増永, 慎一郎 |
Keywords: | quiescent cell hypoxia -rays carbon ion beam boron neutron capture reaction pimonidazole |
Issue Date: | Jan-2013 |
Publisher: | British Institute of Radiology |
Journal title: | British journal of radiology |
Volume: | 86 |
Thesis number: | 20120302 |
Abstract: | [Objectives] To detect the radiosensitivity of intratumour quiescent (Q) cells unlabelled with pimonidazole to accelerated carbon ion beams and the boron neutron capture reaction (BNCR). [Methods] EL4 tumour-bearing C57BL/J mice received 5-bromo-2′-deoxyuridine (BrdU) continuously to label all intratumour proliferating (P) cells. After the administration of pimonidazole, tumours were irradiated with γ-rays, accelerated carbon ion beams or reactor neutron beams with the prior administration of a [10]B-carrier. Responses of intratumour Q and total (P+Q) cell populations were assessed based on frequencies of micronucleation and apoptosis using immunofluorescence staining for BrdU. The response of pimonidazole-unlabelled tumour cells was assessed by means of apoptosis frequency using immunofluorescence staining for pimonidazole. [Results] Following γ-ray irradiation, the pimonidazole-unlabelled tumour cell fraction showed significantly enhanced radiosensitivity compared with the whole tumour cell fraction, more remarkably in the Q than total cell populations. However, a significantly greater decrease in radiosensitivity in the pimonidazole-unlabelled cell fraction, evaluated using a delayed assay or a decrease in radiation dose rate, was more clearly observed among the Q than total cells. These changes in radiosensitivity were suppressed following carbon ion beam and neutron beam-only irradiaton. In the BNCR, the use of a[10]B-carrier, especially L-para-boronophenylalanine-[10]B, enhanced the sensitivity of the pimonidazole-unlabelled cells more clearly in the Q than total cells. [Conclusion] The radiosensitivity of the pimonidazole-unlabelled cell fraction depends on the quality of radiation delivered and characteristics of the [10]B-carrier used in the BNCR. |
Rights: | © 2013 The British Institute of Radiology This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 |
URI: | http://hdl.handle.net/2433/174328 |
DOI(Published Version): | 10.1259/bjr.20120302 |
PubMed ID: | 23255546 |
Appears in Collections: | Journal Articles |

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