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dc.contributor.authorShitan, Nobukazuja
dc.contributor.authorDalmas, Fabienja
dc.contributor.authorDan, Kazuyukija
dc.contributor.authorKato, Nobuhikoja
dc.contributor.authorUeda, Kazumitsuja
dc.contributor.authorSato, Fumihikoja
dc.contributor.authorForestier, Cyrilleja
dc.contributor.authorYazaki, Kazufumija
dc.contributor.alternative矢崎, 一史ja
dc.date.accessioned2013-07-03T01:40:14Z-
dc.date.available2013-07-03T01:40:14Z-
dc.date.issued2013-07ja
dc.identifier.issn0031-9422ja
dc.identifier.urihttp://hdl.handle.net/2433/175501-
dc.description.abstractHigher plants produce a large number of secondary metabolites. Among these are the alkaloids, a group of small nitrogen-containing molecules. Alkaloids often have strong biological activity that protects alkaloid-producing plants from herbivores, and often accumulate to high concentrations in a specific organelle of a particular organ in the producing plant. However, knowledge of the membrane transport mechanism of alkaloids is still limited. Coptis japonica, a perennial Ranunculaceous plant, produces the benzylisoquinoline alkaloid berberine. This alkaloid, though biosynthesized in root tissues, accumulates in the rhizome, suggesting translocation of the molecule via xylem. In this study, a gene encoding a ATP-binding cassette (ABC) protein of B-type, Cjabcb2, was isolated from C. japonica. Northern analysis showed that Cjabcb2 was preferentially expressed in the rhizome, which is the sink organ of berberine. Functional analysis of CjABCB2 using yeast suggested that CjABCB2 transports berberine in an inward direction. Membrane separation and in situ hybridization data indicated that CjABCB2 might be involved in translocation of berberine from the root to the rhizome by transporting berberine at the plasma membrane of cells around the xylem of the rhizome.ja
dc.format.mimetypeapplication/pdfja
dc.language.isoengja
dc.publisherElsevier Ltd.ja
dc.rights© 2012 Elsevier Ltd.ja
dc.rightsThis is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。ja
dc.subjectCoptis japonicaja
dc.subjectRanunculaceaeja
dc.subjectABC transporterja
dc.subjectAlkaloidja
dc.subjectBerberineja
dc.subjectCjABCB2ja
dc.subjectTranslocationja
dc.titleCharacterization of Coptis japonica CjABCB2, an ATP-binding cassette protein involved in alkaloid transport.ja
dc.type.niitypeJournal Articleja
dc.identifier.ncidAA00774558ja
dc.identifier.jtitlePhytochemistryja
dc.identifier.volume91ja
dc.identifier.spage109ja
dc.identifier.epage116ja
dc.relation.doi10.1016/j.phytochem.2012.02.012ja
dc.textversionauthorja
dc.identifier.pmid22410351ja
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