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dc.contributor.author | Minamino, Kento | en |
dc.contributor.author | Takahara, Kazuhiko | en |
dc.contributor.author | Adachi, Takumi | en |
dc.contributor.author | Nagaoka, Koji | en |
dc.contributor.author | Iyoda, Tomonori | en |
dc.contributor.author | Taki, Shinsuke | en |
dc.contributor.author | Inaba, Kayo | en |
dc.contributor.alternative | 稲葉, カヨ | ja |
dc.date.accessioned | 2013-07-19T06:20:42Z | - |
dc.date.available | 2013-07-19T06:20:42Z | - |
dc.date.issued | 2012-07-05 | - |
dc.identifier.issn | 1460-2377 | - |
dc.identifier.uri | http://hdl.handle.net/2433/176343 | - |
dc.description.abstract | Interferon regulatory factor (IRF)-2 is a transcription factor involved in type I (IFN- α/β) signaling. It has been reported that IRF-2 deficiency results in various immune dysfunctions. However, the role of IRF-2 in B-cell functions needs to be elucidated. Unlike wild-type (WT) B cells, IRF-2(-/-) B2 cells were refractory to anti-IgM, but not LPS. Such a defect in proliferation was dependent on IFN- α/β receptor (IFNAR). Marginal zone B cells increased in the proportion relative to B2 cells in IRF-2(-/-) mice produced IgM normally to LPS stimulation. However, IRF-2(-/-) B2 cells were defective in IgM production in an IFNAR-independent manner, although both B-cell subsets differentiated phenotypically to plasma cells at elevated efficiencies. Class switch recombination of IRF-2(-/-) B2 cells by LPS plus IL-4 was also impaired. Their reduced IgM production was conceivably due to an inefficient up-regulation of Blimp-1. Consistent with these in vitro observations, specific antibody production in vivo to a T-dependent antigen by B2 cells was severely impaired in IRF-2(-/- )mice. However, a low, but significant, level of IgG was detected at a late time point, and this IgG exhibited comparable binding affinity to that in WT mice. Follicular helper T-cell development and germinal center formation were normal. A similar tendency was observed when µ chain(-/-) mice were reconstituted with IRF-2(-/- )B cells. These results revealed a multi-faceted role of IRF-2 in the function of B cells, particularly B2 cells, through regulating proliferation in an IFNAR-dependent manner and antibody production via up-regulation of Blimp-1. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Oxford University Press | en |
dc.rights | © The Author 2012. Published by Oxford University Press. | en |
dc.rights | この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 | ja |
dc.rights | This is not the published version. Please cite only the published version. | en |
dc.subject | activation | en |
dc.subject | antibody production | en |
dc.subject | B cells | en |
dc.subject | differentiation | en |
dc.subject | interferon regulatory factor-2 | en |
dc.subject.mesh | Animals | en |
dc.subject.mesh | Antibody Formation/genetics | en |
dc.subject.mesh | B-Lymphocytes/immunology | en |
dc.subject.mesh | B-Lymphocytes/transplantation | en |
dc.subject.mesh | Cell Differentiation/genetics | en |
dc.subject.mesh | Cell Proliferation | en |
dc.subject.mesh | Cells, Cultured | en |
dc.subject.mesh | Gene Expression Regulation | en |
dc.subject.mesh | Immunoglobulin Class Switching/genetics | en |
dc.subject.mesh | Immunoglobulin mu-Chains/genetics | en |
dc.subject.mesh | Interferon Regulatory Factor-2/genetics | en |
dc.subject.mesh | Interferon Regulatory Factor-2/metabolism | en |
dc.subject.mesh | Lipopolysaccharides/immunology | en |
dc.subject.mesh | Mice | en |
dc.subject.mesh | Mice, Inbred C57BL | en |
dc.subject.mesh | Mice, Knockout | en |
dc.subject.mesh | Plasma Cells/immunology | en |
dc.subject.mesh | Plasma Cells/transplantation | en |
dc.subject.mesh | Receptor, Interferon alpha-beta/metabolism | en |
dc.subject.mesh | T-Lymphocytes, Helper-Inducer/immunology | en |
dc.subject.mesh | Transcription Factors/genetics | en |
dc.subject.mesh | Transcription Factors/metabolism | en |
dc.title | IRF-2 regulates B-cell proliferation and antibody production through distinct mechanisms. | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | International immunology | en |
dc.identifier.volume | 24 | - |
dc.identifier.issue | 9 | - |
dc.identifier.spage | 573 | - |
dc.identifier.epage | 581 | - |
dc.relation.doi | 10.1093/intimm/dxs060 | - |
dc.textversion | author | - |
dc.identifier.pmid | 22773153 | - |
dcterms.accessRights | open access | - |
出現コレクション: | 学術雑誌掲載論文等 |
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