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Title: Manufacture of a weakly denatured collagen fiber scaffold with excellent biocompatibility and space maintenance ability.
Authors: Nakada, A
Shigeno, K
Sato, T
Kobayashi, T
Wakatsuki, M
Uji, M
Nakamura, T
Author's alias: 中田, 顕
Issue Date: 27-Jun-2013
Publisher: IOP Publishing
Journal title: Biomedical materials
Volume: 8
Issue: 4
Thesis number: 45010
Abstract: Although collagen scaffolds have been used for regenerative medicine, they have insufficient mechanical strength. We made a weakly denatured collagen fiber scaffold from a collagen fiber suspension (physiological pH 7.4) through a process of freeze drying and denaturation with heat under low pressure (1 × 10(-1) Pa). Heat treatment formed cross-links between the collagen fibers, providing the scaffold with sufficient mechanical strength to maintain the space for tissue regeneration in vivo. The scaffold was embedded under the back skin of a rat, and biocompatibility and space maintenance ability were examined after 2 weeks. These were evaluated by using the ratio of foreign body giant cells and thickness of the residual scaffold. A weakly denatured collagen fiber scaffold with moderate biocompatibility and space maintenance ability was made by freezing at -10 °C, followed by denaturation at 140 °C for 6 h. In addition, the direction of the collagen fibers in the scaffold was adjusted by cooling the suspension only from the bottom of the container. This process increased the ratio of cells that infiltrated into the scaffold. A weakly denatured collagen fiber scaffold thus made can be used for tissue regeneration or delivery of cells or proteins to a target site.
Rights: © 2013 IOP Publishing
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
DOI(Published Version): 10.1088/1748-6041/8/4/045010
PubMed ID: 23804650
Appears in Collections:Journal Articles

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