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j.jcrysgro.2013.01.003.pdf | 212.31 kB | Adobe PDF | 見る/開く |
タイトル: | Observation of the orientation of membrane protein crystals grown in high magnetic force fields |
著者: | Numoto, Nobutaka Shimizu, Ken-ichi Matsumoto, Kazuya Miki, Kunio ![]() Kita, Akiko ![]() ![]() ![]() |
著者名の別形: | 喜田, 昭子 |
キーワード: | A1. Magnetic fields B1. Biological macromolecules B3. Superconducting magnet |
発行日: | 15-Mar-2013 |
出版者: | Elsevier B.V. |
誌名: | Journal of Crystal Growth |
巻: | 367 |
開始ページ: | 53 |
終了ページ: | 56 |
抄録: | Crystallization of membrane proteins in magnetic fields is thought to reveal the magnetic orientations of crystals, and is expected to enhance crystal quality for X-ray crystallographic analysis. The light-harvesting complex 2 (LH2) from a photosynthetic bacterium, Thermochromatium tepidum was crystallized in steep-gradient magnetic fields. The rod-shaped crystals of LH2 grown in the magnetic fields were oriented parallel to the magnetic field direction. An X-ray diffraction experiment indicated that the overall R value and crystal mosaicity are improved for the magnetically oriented crystal, and the helix bundles of LH2 were located parallel to the magnetic field direction in the crystal packing. |
著作権等: | © 2013 Elsevier B.V. This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 |
URI: | http://hdl.handle.net/2433/178065 |
DOI(出版社版): | 10.1016/j.jcrysgro.2013.01.003 |
出現コレクション: | 学術雑誌掲載論文等 |

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