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Title: ARF1 and ARF4 regulate recycling endosomal morphology and retrograde transport from endosomes to the Golgi apparatus.
Authors: Nakai, Waka
Kondo, Yumika
Saitoh, Akina
Naito, Tomoki
Nakayama, Kazuhisa  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0001-7701-7183 (unconfirmed)
Shin, Hye-Won  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-9138-9554 (unconfirmed)
Author's alias: 申, 惠媛
Issue Date: Aug-2013
Publisher: American Society for Cell Biology
Journal title: Molecular biology of the cell
Volume: 24
Issue: 16
Start page: 2570
End page: 2581
Abstract: Small GTPases of the ADP-ribosylation factor (ARF) family, except for ARF6, mainly localize to the Golgi apparatus, where they trigger formation of coated carrier vesicles. We recently showed that class I ARFs (ARF1 and ARF3) localize to recycling endosomes, as well as to the Golgi, and are redundantly required for recycling of endocytosed transferrin. On the other hand, the roles of class II ARFs (ARF4 and ARF5) are not yet fully understood, and the complementary or overlapping functions of class I and class II ARFs have been poorly characterized. In this study, we find that simultaneous depletion of ARF1 and ARF4 induces extensive tubulation of recycling endosomes. Moreover, the depletion of ARF1 and ARF4 inhibits retrograde transport of TGN38 and mannose-6-phosphate receptor from early/recycling endosomes to the trans-Golgi network (TGN) but does not affect the endocytic/recycling pathway of transferrin receptor or inhibit retrograde transport of CD4-furin from late endosomes to the TGN. These observations indicate that the ARF1+ARF4 and ARF1+ARF3 pairs are both required for integrity of recycling endosomes but are involved in distinct transport pathways: the former pair regulates retrograde transport from endosomes to the TGN, whereas the latter is required for the transferrin recycling pathway from endosomes to the plasma membrane.
Rights: © 2013 Nakai et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).
URI: http://hdl.handle.net/2433/178753
DOI(Published Version): 10.1091/mbc.E13-04-0197
PubMed ID: 23783033
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