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dc.contributor.author | Nakamichi, Yusuke | en |
dc.contributor.author | Yoshioka, Aya | en |
dc.contributor.author | Kawai, Shigeyuki | en |
dc.contributor.author | Murata, Kousaku | en |
dc.contributor.alternative | 中道, 優介 | ja |
dc.contributor.alternative | 吉岡, 彩 | ja |
dc.contributor.alternative | 河井, 重幸 | ja |
dc.contributor.alternative | 村田, 幸作 | ja |
dc.date.accessioned | 2013-09-27T05:30:34Z | - |
dc.date.available | 2013-09-27T05:30:34Z | - |
dc.date.issued | 2013-09-11 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | http://hdl.handle.net/2433/178764 | - |
dc.description | ATP特異性の獲得メカニズムの解明 : 新薬と新しい物質生産系の開発に期待. 京都大学プレスリリース. 2013-09-11. | ja |
dc.description.abstract | NAD kinase (NADK) is a crucial enzyme for production of NADP(+). ATP-specific NADK prefers ATP to inorganic polyphosphate [poly(P)] as a phosphoryl donor, whereas poly(P)/ATP-NADK utilizes both ATP and poly(P), and is employed in industrial mass production of NADP(+). Poly(P)/ATP-NADKs are distributed throughout Gram-positive bacteria and Archaea, whereas ATP-specific NADKs are found in Gram-negative α- and γ-proteobacteria and eukaryotes. In this study, we succeeded in conferring the ability to utilize poly(P) on γ-proteobacterial ATP-specific NADKs through a single amino-acid substitution; the substituted amino-acid residue is therefore important in determining the phosphoryl-donor specificity of γ-proteobacterial NADKs. We also demonstrate that a poly(P)/ATP-NADK created through this method is suitable for the poly(P)-dependent mass production of NADP(+). Moreover, based on our results, we provide insight into the evolution of bacterial NADKs, in particular, how NADKs evolved from poly(P)/ATP-NADKs into ATP-specific NADKs. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Nature Publishing Group | en |
dc.rights | © 2013 Nature Publishing Group | en |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/ | en |
dc.subject | Molecular evolution | en |
dc.subject | Enzyme mechanisms | en |
dc.subject | Kinases | en |
dc.subject | Protein design | en |
dc.title | Conferring the ability to utilize inorganic polyphosphate on ATP-specific NAD kinase | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Scientific reports | en |
dc.identifier.volume | 3 | - |
dc.relation.doi | 10.1038/srep02632 | - |
dc.textversion | publisher | - |
dc.identifier.artnum | 2632 | - |
dc.identifier.pmid | 24022322 | - |
dc.relation.url | https://www.kyoto-u.ac.jp/static/ja/news_data/h/h1/news6/2013/130911_1.htm | - |
dcterms.accessRights | open access | - |
出現コレクション: | 学術雑誌掲載論文等 |
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