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Title: Differentiation-defective phenotypes revealed by large-scale analyses of human pluripotent stem cells.
Authors: Koyanagi-Aoi, Michiyo
Ohnuki, Mari
Takahashi, Kazutoshi  kyouindb  KAKEN_id
Okita, Keisuke  kyouindb  KAKEN_id  orcid (unconfirmed)
Noma, Hisashi
Sawamura, Yuka
Teramoto, Ito
Narita, Megumi
Sato, Yoshiko
Ichisaka, Tomoko
Amano, Naoki
Watanabe, Akira  kyouindb  KAKEN_id  orcid (unconfirmed)
Morizane, Asuka  kyouindb  KAKEN_id  orcid (unconfirmed)
Yamada, Yasuhiro  kyouindb  KAKEN_id
Sato, Tosiya  orcid (unconfirmed)
Takahashi, Jun  kyouindb  KAKEN_id
Yamanaka, Shinya  kyouindb  KAKEN_id
Author's alias: 青井, 三千代
大貫, 茉里
高橋, 和利
沖田, 圭介
野間, 久史
澤村, 由香
寺本, 一糸
成田, 恵
佐藤, 美子
一阪, 朋子
天野, 直己
渡辺, 亮
森実, 飛鳥
山田, 泰広
佐藤, 俊哉
高橋, 淳
山中, 伸弥
Issue Date: 20-Nov-2013
Publisher: National Academy of Sciences
Journal title: Proceedings of the National Academy of Sciences of the United States of America
Volume: 110
Issue: 51
Start page: 20569
End page: 20574
Abstract: We examined the gene expression and DNA methylation of 49 human induced pluripotent stem cells (hiPSCs) and 10 human embryonic stem cells and found overlapped variations in gene expression and DNA methylation in the two types of human pluripotent stem cell lines. Comparisons of the in vitro neural differentiation of 40 hiPSCs and 10 human embryonic stem cells showed that seven hiPSC clones retained a significant number of undifferentiated cells even after neural differentiation culture and formed teratoma when transplanted into mouse brains. These differentiation-defective hiPSC clones were marked by higher expression levels of several genes, including those expressed from long terminal repeats of specific human endogenous retroviruses. These data demonstrated a subset of hiPSC lines that have aberrant gene expression and defective potential in neural differentiation, which need to be identified and eliminated before applications in regenerative medicine.
Description: 大規模解析により品質の悪い多能性幹細胞の見分け方を開発. 京都大学プレスリリース. 2013-11-19.
Rights: © 2013 National Academy of Sciences
This is not the published version. Please cite only the published version.
DOI(Published Version): 10.1073/pnas.1319061110
PubMed ID: 24259714
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