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タイトル: | Factors Influencing the Surface Modification of Mesenchymal Stem Cells with Fluorescein-Pegylated Lipids |
著者: | Takafuji, Yoshimasa Higuchi, Yuriko https://orcid.org/0000-0002-9869-1019 (unconfirmed) Muro, Atsushi Oshiro, Kohei Kawakami, Shigeru Yamashita, Fumiyoshi https://orcid.org/0000-0002-3503-8696 (unconfirmed) Hashida, Mitsuru |
著者名の別形: | 樋口, ゆり子 |
キーワード: | pegylated lipid mesenchymal stem cell cell surface modification cell therapy |
発行日: | 1-Nov-2013 |
出版者: | Pharmaceutical Society of Japan |
誌名: | Biological and Pharmaceutical Bulletin |
巻: | 36 |
号: | 11 |
開始ページ: | 1731 |
終了ページ: | 1738 |
抄録: | Artificial introduction of functional molecules on the cell surface may be a promising way to improve the therapeutic effects of cell therapy. Pegylated lipids are conventionally used in drug carriers. The lipid part of pegylated lipids noncovalently interacts with the cell surface. However, little information is available regarding conditions for cell-surface modification by using pegylated lipids. In this study, we synthesized fluorescein-labeled pegylated lipids and evaluated the factors that affect modification efficiency by using human mesenchymal stem cells (hMSCs). As the concentration of the pegylated lipid as well as the exposure time increased, the modification efficiency increased. The modification efficiency at 37°C was 20- and 3-fold higher than that at 4°C and 25°C, respectively. In addition, with an increase in the molecular weight of polyethylene glycol (PEG), more pegylated lipids were extracellularly distributed than those intracellularly distributed. At the optimal condition, pegylated lipids were observed mainly on the cell membrane by confocal microscopy. In contrast, the cell condition (adherent or nonadherent) had little or no effect on the cell-surface modification efficiency. The results of this study will be useful for constructing an optimal modification method for introducing functional molecules on the cell surface. |
著作権等: | © 2013 The Pharmaceutical Society of Japan |
URI: | http://hdl.handle.net/2433/179768 |
DOI(出版社版): | 10.1248/bpb.b13-00291 |
PubMed ID: | 24189418 |
出現コレクション: | 学術雑誌掲載論文等 |
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