ダウンロード数: 452
このアイテムのファイル:
ファイル | 記述 | サイズ | フォーマット | |
---|---|---|---|---|
j.jconrel.2013.10.024.pdf | 457.73 kB | Adobe PDF | 見る/開く |
完全メタデータレコード
DCフィールド | 値 | 言語 |
---|---|---|
dc.contributor.author | Kusamori, Kosuke | en |
dc.contributor.author | Nishikawa, Makiya | en |
dc.contributor.author | Mizuno, Narumi | en |
dc.contributor.author | Nishikawa, Tomoko | en |
dc.contributor.author | Masuzawa, Akira | en |
dc.contributor.author | Shimizu, Kazunori | en |
dc.contributor.author | Konishi, Satoshi | en |
dc.contributor.author | Takahashi, Yuki | en |
dc.contributor.author | Takakura, Yoshinobu | en |
dc.contributor.alternative | 西川, 元也 | ja |
dc.date.accessioned | 2013-12-25T07:06:37Z | - |
dc.date.available | 2013-12-25T07:06:37Z | - |
dc.date.issued | 2014-01-10 | - |
dc.identifier.issn | 0168-3659 | - |
dc.identifier.uri | http://hdl.handle.net/2433/179777 | - |
dc.description.abstract | The efficacy of cell-based therapy depends on the function and survival of transplanted cells, which have been suggested to be enhanced by spheroid formation. However, few attempts at spheroid generation from insulin-secreting cells, which may be used to treat type 1 diabetes, have been reported. We therefore developed spheroids from the mouse insulinoma cell line NIT-1 by using polydimethylsiloxane (PDMS)-based microwells with a coating of poly(N-isopropylacrylamide) (PNIPAAm). The prepared NIT-1 spheroids or dissociated NIT-1 cells were transplanted into the subrenal capsule in streptozotocin-induced diabetic mice. NIT-1 spheroids prepared using the PNIPAAm-coated PDMS-based microwells had a uniformly sized spherical structure with a diameter of 200-300μm. The PNIPAAm coating increased cell survival in the spheroids and the recovery of the spheroids from the microwells. In diabetic mice, the transplanted NIT-1 spheroids reduced blood glucose levels to normal values faster than dissociated NIT-1 cells did. Additionally, survival was higher among NIT-1 cells in spheroids than among dissociated NIT-1 cells 24h after transplantation. These results indicate that insulin-secreting NIT-1 spheroids prepared using PNIPAAm-coated PDMS-based microwells are more effective for the treatment of type 1 diabetes than dissociated cells in suspension. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier BV | en |
dc.rights | © 2013 Elsevier B.V. | en |
dc.rights | This is not the published version. Please cite only the published version. | en |
dc.rights | この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 | ja |
dc.subject | Allogeneic cell | en |
dc.subject | Cell viability | en |
dc.subject | Diabetes | en |
dc.subject | Multicellular spheroid | en |
dc.subject | Silicone elastomer | en |
dc.subject | Transplantation | en |
dc.title | Transplantation of insulin-secreting multicellular spheroids for the treatment of type 1 diabetes in mice. | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.ncid | AA10458678 | - |
dc.identifier.jtitle | Journal of controlled release | en |
dc.identifier.volume | 173 | - |
dc.identifier.spage | 119 | - |
dc.identifier.epage | 124 | - |
dc.relation.doi | 10.1016/j.jconrel.2013.10.024 | - |
dc.textversion | author | - |
dc.identifier.pmid | 24184345 | - |
dcterms.accessRights | open access | - |
dc.identifier.pissn | 0168-3659 | - |
dc.identifier.eissn | 1873-4995 | - |
出現コレクション: | 学術雑誌掲載論文等 |
このリポジトリに保管されているアイテムはすべて著作権により保護されています。