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Title: Micelle-based activatable probe for in vivo near-infrared optical imaging of cancer biomolecules.
Authors: Shimizu, Yoichi  kyouindb  KAKEN_id
Temma, Takashi
Hara, Isao
Makino, Akira
Yamahara, Ryo
Ozeki, Ei-Ichi
Ono, Masahiro  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-2497-039X (unconfirmed)
Saji, Hideo  kyouindb  KAKEN_id
Author's alias: 天滿, 敬
佐治, 英郎
Keywords: Lactosome
Polymeric micelle
Near-infrared fluorescence imaging
Activatable probe
Molecular imaging
Issue Date: Jan-2014
Publisher: Elsevier BV
Journal title: Nanomedicine : nanotechnology, biology, and medicine
Volume: 10
Issue: 1
Start page: 187
End page: 195
Abstract: Near-infrared (NIR: 800-1000nm) fluorescent probes, which activate their fluorescence following interaction with functional biomolecules, are desirable for noninvasive and sensitive tumor diagnosis due to minimal tissue interference. Focusing on bioavailability and applicability, we developed a probe with a self-assembling polymer micelle, a lactosome, encapsulating various quantities of NIR dye (IC7-1). We also conjugated anti-HER2 single chain antibodies to the lactosome surface and examined the probe's capacity to detect HER2 in cells and in vivo. Micelles encapsulating 20mol% IC7-1 (hIC7L) showed 30-fold higher fluorescence (λem: 858nm) after micelle denaturation compared to aqueous buffer. Furthermore, antibody modification allowed specific activation of the probe (HER2-hIC7L) following internalization by HER2-positive cells, with the probe concentrating in lysosomes. HER2-hIC7L intravenously administered to mice clearly and specifically visualized HER2-positive tumors by in vivo optical imaging. These results indicate that HER2-hIC7L is a potential activatable NIR probe for sensitive tumor diagnosis.
Rights: © 2014 Elsevier Inc.
This is not the published version. Please cite only the published version.
この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/180295
DOI(Published Version): 10.1016/j.nano.2013.06.009
PubMed ID: 23811292
Appears in Collections:Journal Articles

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