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j.nano.2013.06.009.pdf | 344.11 kB | Adobe PDF | 見る/開く |
タイトル: | Micelle-based activatable probe for in vivo near-infrared optical imaging of cancer biomolecules. |
著者: | Shimizu, Yoichi Temma, Takashi Hara, Isao Makino, Akira Yamahara, Ryo Ozeki, Ei-Ichi Ono, Masahiro https://orcid.org/0000-0002-2497-039X (unconfirmed) Saji, Hideo |
著者名の別形: | 天滿, 敬 佐治, 英郎 |
キーワード: | Lactosome Polymeric micelle Near-infrared fluorescence imaging Activatable probe Molecular imaging |
発行日: | Jan-2014 |
出版者: | Elsevier BV |
誌名: | Nanomedicine : nanotechnology, biology, and medicine |
巻: | 10 |
号: | 1 |
開始ページ: | 187 |
終了ページ: | 195 |
抄録: | Near-infrared (NIR: 800-1000nm) fluorescent probes, which activate their fluorescence following interaction with functional biomolecules, are desirable for noninvasive and sensitive tumor diagnosis due to minimal tissue interference. Focusing on bioavailability and applicability, we developed a probe with a self-assembling polymer micelle, a lactosome, encapsulating various quantities of NIR dye (IC7-1). We also conjugated anti-HER2 single chain antibodies to the lactosome surface and examined the probe's capacity to detect HER2 in cells and in vivo. Micelles encapsulating 20mol% IC7-1 (hIC7L) showed 30-fold higher fluorescence (λem: 858nm) after micelle denaturation compared to aqueous buffer. Furthermore, antibody modification allowed specific activation of the probe (HER2-hIC7L) following internalization by HER2-positive cells, with the probe concentrating in lysosomes. HER2-hIC7L intravenously administered to mice clearly and specifically visualized HER2-positive tumors by in vivo optical imaging. These results indicate that HER2-hIC7L is a potential activatable NIR probe for sensitive tumor diagnosis. |
著作権等: | © 2014 Elsevier Inc. This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 |
URI: | http://hdl.handle.net/2433/180295 |
DOI(出版社版): | 10.1016/j.nano.2013.06.009 |
PubMed ID: | 23811292 |
出現コレクション: | 学術雑誌掲載論文等 |
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