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タイトル: | Transcriptional regulatory factor X6 (Rfx6) increases gastric inhibitory polypeptide (GIP) expression in enteroendocrine K-cells and is involved in GIP hypersecretion in high fat diet-induced obesity. |
著者: | Suzuki, Kazuyo https://orcid.org/0000-0001-5716-5242 (unconfirmed) Harada, Norio Yamane, Shunsuke Nakamura, Yasuhiko Sasaki, Kazuki Nasteska, Daniela Joo, Erina Shibue, Kimitaka Harada, Takanari Hamasaki, Akihiro Toyoda, Kentaro Nagashima, Kazuaki Inagaki, Nobuya |
著者名の別形: | 鈴木, 和代 稲垣, 暢也 |
キーワード: | Gene Expression Hormones Intestine Nutrition Obesity GIP K-cell Pdx1 Rfx6 |
発行日: | 18-Jan-2013 |
出版者: | American Society for Biochemistry and Molecular Biology |
誌名: | The Journal of biological chemistry |
巻: | 288 |
号: | 3 |
開始ページ: | 1929 |
終了ページ: | 1938 |
抄録: | Gastric inhibitory polypeptide (GIP) is an incretin released from enteroendocrine K-cells in response to nutrient ingestion. GIP potentiates glucose-stimulated insulin secretion and induces energy accumulation into adipose tissue, resulting in obesity. Plasma GIP levels are reported to be increased in the obese state. However, the molecular mechanisms of GIP secretion and high fat diet (HFD)-induced GIP hypersecretion remain unclear, primarily due to difficulties in separating K-cells from other intestinal epithelial cells in vivo. In this study, GIP-GFP knock-in mice that enable us to visualize K-cells by enhanced GFP were established. Microarray analysis of isolated K-cells from these mice revealed that transcriptional regulatory factor X6 (Rfx6) is expressed exclusively in K-cells. In vitro experiments using the mouse intestinal cell line STC-1 showed that knockdown of Rfx6 decreased mRNA expression, cellular content, and secretion of GIP. Rfx6 bound to the region in the gip promoter that regulates gip promoter activity, and overexpression of Rfx6 increased GIP mRNA expression. HFD induced obesity and GIP hypersecretion in GIP-GFP heterozygous mice in vivo. Immunohistochemical and flow cytometry analysis showed no significant difference in K-cell number between control fat diet-fed (CFD) and HFD-fed mice. However, GIP content in the upper small intestine and GIP mRNA expression in K-cells were significantly increased in HFD-fed mice compared with those in CFD-fed mice. Furthermore, expression levels of Rfx6 mRNA were increased in K-cells of HFD-fed mice. These results suggest that Rfx6 increases GIP expression and content in K-cells and is involved in GIP hypersecretion in HFD-induced obesity. |
著作権等: | © 2013 by The American Society for Biochemistry and Molecular Biology, Inc. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/182055 |
DOI(出版社版): | 10.1074/jbc.M112.423137 |
PubMed ID: | 23192339 |
出現コレクション: | 学術雑誌掲載論文等 |
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