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タイトル: | Regulation of pH attenuates toxicity of a byproduct produced by an ethanologenic strain of Sphingomonas sp. A1 during ethanol fermentation from alginate |
著者: | Fujii, Mari Yoshida, Shiori Murata, Kousaku Kawai, Shigeyuki |
著者名の別形: | 村田, 幸作 河井, 重幸 |
キーワード: | ethanol alginate mannitol Sphingomonas Saccharomyces paradoxus |
発行日: | 9-Jan-2014 |
出版者: | Landes Bioscience |
誌名: | Bioengineered |
巻: | 5 |
号: | 1 |
開始ページ: | 1 |
終了ページ: | 7 |
抄録: | Marine macroalgae is a promising carbon source that contains alginate and mannitol as major carbohydrates. A bioengineered ethanologenic strain of the bacterium Sphingomonas sp. A1 can produce ethanol from alginate, but not mannitol, whereas the yeast Saccharomyces paradoxus NBRC 0259–3 can produce ethanol from mannitol, but not alginate. Thus, one practical approach for converting both alginate and mannitol into ethanol would involve two-step fermentation, in which the ethanologenic bacterium initially converts alginate into ethanol, and then the yeast produces ethanol from mannitol. In this study, we found that, during fermentation from alginate, the ethanologenic bacterium lost viability and secreted toxic byproducts into the medium. These toxic byproducts inhibited bacterial growth and killed bacterial cells and also inhibited growth of S. paradoxus NBRC 0259–3. We discovered that adjusting the pH of the culture supernatant or the culture medium containing the toxic byproducts to 6.0 attenuated the toxicity toward both bacteria and yeast, and also extended the period of viability of the bacterium. Although continuous adjustment of pH to 6.0 failed to improve the ethanol productivity of this ethanologenic bacterium, this pH adjustment worked very well in the two-step fermentation due to the attenuation of toxicity toward S. paradoxus NBRC 0259–3. These findings provide information critical for establishment of a practical system for ethanol production from brown macroalgae. |
著作権等: | © 2014 Landes Bioscience This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 |
URI: | http://hdl.handle.net/2433/182902 |
DOI(出版社版): | 10.4161/bioe.27397 |
PubMed ID: | 24445222 |
出現コレクション: | 学術雑誌掲載論文等 |
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