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Title: Light-dependent destabilization of PHL in Arabidopsis
Authors: Endo, Motomu
Kudo, Daiki
Koto, Tomoko
Shimizu, Hanako
Araki, Takashi  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-3616-1493 (unconfirmed)
Author's alias: 遠藤, 求
Keywords: Arabidopsis
flowering
florigen
phytochrome
photoperiod
FT
CO
phyB
PHL
Issue Date: 10-Mar-2014
Publisher: Landes Bioscience
Journal title: Plant Signaling & Behavior
Volume: 9
Issue: 2
Thesis number: e28118
Abstract: Plants sense environmental stimuli such as light to regulate their flowering time. In Arabidopsis, phytochrome B (phyB) is the major photoreceptor that perceives red and far-red light, and destabilizes transcriptional regulator CONSTANS (CO) protein. However the mechanism that links photoreceptor and CO protein degradation is largely unknown. We recently showed that PHYTOCHROME-DEPENDENT LATE-FLOWERING (PHL) protein inhibits phyB signaling through direct protein-protein interaction. Here, we report that light exposure destabilizes PHL protein as is the case with CO. Fluorescence from PHL-YFP fusion protein expressed under the control of Cauliflower Mosaic Virus (CaMV) 35S promoter (35S::PHL-YFP) almost disappeared after four-hour treatment of white light. Furthermore, the similar results were also obtained from the analysis of PHL-GUS fusion protein expressed by PHL promoter (PHLpro::PHL-GUS phl-1). These results highlight the importance of post-transcriptional regulation in phyB-mediated flowering regulation and will give us hints how phyB regulates CO protein amount.
Rights: © 2014 Landes Bioscience
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/187356
DOI(Published Version): 10.4161/psb.28118
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