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Title: The dependency of compound biological effectiveness factors on the type and the concentration of administered neutron capture agents in boron neutron capture therapy
Authors: Masunaga, Shin-ichiro  kyouindb  KAKEN_id
Sakurai, Yoshinori
Tanaka, Hiroki  kyouindb  KAKEN_id
Tano, Keizo  kyouindb  KAKEN_id
Suzuki, Minoru  kyouindb  KAKEN_id
Kondo, Natsuko  kyouindb  KAKEN_id
Narabayashi, Masaru
Nakagawa, Yosuke
Watanabe, Tsubasa  kyouindb  KAKEN_id
Maruhashi, Akira
Ono, Koji
Author's alias: 増永, 慎一郎
Keywords: Boron neutron capture therapy
Neutron capture agent
Relative biological effectiveness
Compound biological effectiveness
Tumor heterogeneity
Quiescent cell
Issue Date: 7-Mar-2014
Publisher: SpringerOpen
Journal title: SpringerPlus
Volume: 3
Issue: 1
Thesis number: 128
Abstract: [Purpose]To examine the effect of the type and the concentration of neutron capture agents on the values of compound biological effectiveness (CBE) in boron neutron capture therapy. [Methods and materials]After the subcutaneous administration of a 10B-carrier, boronophenylalanine-10B (BPA) or sodium mercaptododecaborate-10B (BSH), at 3 separate concentrations, the 10B concentrations in tumors were measured by γ-ray spectrometry. SCC VII tumor-bearing C3H/He mice received 5-bromo-2′-deoxyuridine (BrdU) continuously to label all intratumor proliferating (P) cells, then treated with BPA or BSH. Immediately after reactor neutron beam irradiation, during which intratumor 10B concentrations were kept at levels similar to each other, cells from some tumors were isolated and incubated with a cytokinesis blocker. The responses of BrdU-unlabeled quiescent (Q) and total (= P + Q) tumor cells were assessed based on the frequencies of micronucleation using immunofluorescence staining for BrdU. [Results] The CBE values were higher in Q cells and in the use of BPA than total cells and BSH, respectively. In addition, the higher the administered concentrations were, the smaller the CBE values became, with a clearer tendency in the use of BPA than BSH. The values for neutron capture agents that deliver into solid tumors more dependently on uptake capacity of tumor cells became more changeable. [Conclusion]Tumor characteristics, such as micro-environmental heterogeneity, stochastic genetic or epigenetic changes, or hierarchical organization of tumor cells, are thought to partially influence on the value of CBE, meaning that the CBE value itself may be one of the indices showing the degree of tumor heterogeneity.
Rights: © 2014 Masunaga et al.; licensee Springer.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.
URI: http://hdl.handle.net/2433/187377
DOI(Published Version): 10.1186/2193-1801-3-128
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