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Title: Distribution pattern of GFP (green fluorescent protein) in a bivalve-inhabiting hydrozoan, Eutima japonica (Leptomedusae: Eirenidae)
Authors: Kubota, Shin  kyouindb  KAKEN_id
Nomaru, Eriko
Uchida, Hiroko
Murakami, Akio
Author's alias: 久保田, 信
Keywords: fluorescence pattern and spectra
green fluorescent protein (GFP)
hydromedusa
Eutima
Eugymnanthea
systematics
Issue Date: 14-Jul-2010
Publisher: Cambridge University Press
Journal title: Journal of the Marine Biological Association of the United Kingdom
Volume: 90
Issue: 7
Start page: 1371
End page: 1374
Abstract: Bright green auto-fluorescence was observed in the umbrellar margin, umbrellar marginal warts, tentacular bulbs, tentacles, and manubrium of laboratory-reared immature (1–14 days old) medusae of Eutima japonica from Japan and China. In vivo microscopic fluorescence spectra showed that the green fluorescence was similar to that of green fluorescent protein (GFP) found in Aequorea victoria, although the maximum emission wavelength (503 nm) was slightly bluer. No fluorescence was detected in the cirri, statocysts, radial canals, velum, or subumbrella of the medusae. The fluorescence distribution pattern in E. japonica more closely resembles that of Eugymnanthea inquilina from the Mediterranean Sea than that of Japanese Eugymnanthea japonica, which is the derived species of E. japonica. This suggests that the common fluorescence pattern is convergently evolved in the former two species, perhaps owing to the as yet unclarified physiological and/or ecological function of GFP and/or GFP-like proteins.
Rights: © Marine Biological Association of the United Kingdom 2010
URI: http://hdl.handle.net/2433/187920
DOI(Published Version): 10.1017/S0025315409991500
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