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Title: Community structure of planktonic methane-oxidizing bacteria in a subtropical reservoir characterized by dominance of phylotype closely related to nitrite reducer
Authors: Kojima, Hisaya
Tokizawa, Riho
Kogure, Kouhei
Kobayashi, Yuki
Itoh, Masayuki  kyouindb  KAKEN_id
Shiah, Fuh-Kwo
Okuda, Noboru
Fukui, Manabu
Author's alias: 伊藤, 雅之
夏, 復國
奥田, 昇
福井, 学
Keywords: Water microbiology
Issue Date: 25-Jul-2014
Publisher: Nature Publishing Group
Journal title: Scientific Reports
Volume: 4
Thesis number: 5728
Abstract: Methane-oxidizing bacteria (MOB) gain energy from the oxidation of methane and may play important roles in freshwater ecosystems. In this study, the community structure of planktonic MOB was investigated in a subtropical reservoir. Bacterial community structure was investigated through the analysis of the 16S rRNA gene. Three groups of phylogenetically distinct MOB were detected in the clone libraries of polymerase chain reaction products obtained with universal primers. The groups belonged to the class Gammaproteobacteria, the class Alphaproteobacteria, and the candidate phylum NC10. The last group, which consists of close relatives of the nitrite reducer 'Candidatus Methylomirabilis oxyfera', was frequently detected in the clone libraries of deep-water environments. The presence of 3 groups of MOB in deep water was also shown by a cloning analysis of the pmoA gene encoding particulate methane monooxygenase. The dominance of 'M. oxyfera'-like organisms in deep water was confirmed by catalyzed reporter deposition–fluorescence in situ hybridization, in which cells stained with a specific probe accounted for 16% of total microbial cells. This is the first study to demonstrate that close relatives of the nitrite reducer can be major component of planktonic MOB community which may affect carbon flow in aquatic ecosystems.
Description: 湖水中でのメタン消費の新たな主役 -亜熱帯ダム湖で脱窒メタン酸化細菌が優占していることを発見-. 京都大学プレスリリース. 2014-07-31.
Rights: This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit
DOI(Published Version): 10.1038/srep05728
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