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j.bmc.2014.04.052.pdf | 275.54 kB | Adobe PDF | 見る/開く |
タイトル: | Design and synthesis of fluorescent probes for GPR54. |
著者: | Kaneda, Masato Misu, Ryosuke Ohno, Hiroaki https://orcid.org/0000-0002-3246-4809 (unconfirmed) Hirasawa, Akira https://orcid.org/0000-0001-5692-805X (unconfirmed) Ieda, Nahoko Uenoyama, Yoshihisa Tsukamura, Hiroko Maeda, Kei-ichiro Oishi, Shinya https://orcid.org/0000-0002-2833-2539 (unconfirmed) Fujii, Nobutaka |
著者名の別形: | 大石, 真也 |
キーワード: | Fluorescent probe GPR54 Kisspeptin RFamide |
発行日: | 1-Jul-2014 |
出版者: | Elsevier BV |
誌名: | Bioorganic & medicinal chemistry |
巻: | 22 |
号: | 13 |
開始ページ: | 3325 |
終了ページ: | 3330 |
抄録: | Kisspeptins are neuropeptides that induce the secretion of gonadotropin-releasing hormone via the activation of the cognate receptor, G-protein coupled receptor 54 (GPR54). The kisspeptin-GPR54 axis is associated with the onset of puberty and the maintenance of the reproductive system. In this study, several fluorescent probes have been designed and synthesized for rat GPR54 through the modification of the N-terminus of rat kisspeptins to allow for the visualization of the expression and localization of kisspeptin receptor(s) in living cells and native tissues. The tetramethylrhodamine (TMR) and rhodamine green (RG)-labeled kisspeptins exhibited good binding and agonistic activities towards GPR54, and the results of the application studies demonstrated that these fluorescent probes could be used effectively for the detection of GPR54 receptors in flow cytometry and confocal microscopy experiments. |
著作権等: | © 2014 Elsevier Ltd. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/189467 |
DOI(出版社版): | 10.1016/j.bmc.2014.04.052 |
PubMed ID: | 24857775 |
出現コレクション: | 学術雑誌掲載論文等 |
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