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dc.contributor.author | Shimizu, Yoichi | en |
dc.contributor.author | Temma, Takashi | en |
dc.contributor.author | Hara, Isao | en |
dc.contributor.author | Makino, Akira | en |
dc.contributor.author | Kondo, Naoya | en |
dc.contributor.author | Ozeki, Ei-Ichi | en |
dc.contributor.author | Ono, Masahiro | en |
dc.contributor.author | Saji, Hideo | en |
dc.contributor.alternative | 天滿, 敬 | ja |
dc.contributor.alternative | 佐治, 英郎 | ja |
dc.date.accessioned | 2014-09-19T07:52:58Z | - |
dc.date.available | 2014-09-19T07:52:58Z | - |
dc.date.issued | 2014-08 | - |
dc.identifier.issn | 1349-7006 | - |
dc.identifier.uri | http://hdl.handle.net/2433/189747 | - |
dc.description.abstract | Membrane type-1 matrix metalloproteinase (MT1-MMP) is a protease activating MMP-2 that mediates cleavage of extracellular matrix components and plays pivotal roles in tumor migration, invasion and metastasis. Because in vivo noninvasive imaging of MT1-MMP would be useful for tumor diagnosis, we developed a novel near-infrared (NIR) fluorescence probe that can be activated following interaction with MT1-MMP in vivo. MT1-hIC7L is an activatable fluorescence probe comprised of anti-MT1-MMP monoclonal antibodies conjugated to self-assembling polymer micelles that encapsulate NIR dyes (IC7-1, λem : 858 nm) at concentrations sufficient to cause fluorescence self-quenching. In aqueous buffer, MT1-hIC7L fluorescence was suppressed to background levels and increased approximately 35.5-fold in the presence of detergent. Cellular uptake experiments revealed that in MT1-MMP positive C6 glioma cells, MT1-hIC7L showed significantly higher fluorescence that increased with time as compared to hIC7L, a negative control probe lacking the anti-MT1-MMP monoclonal antibody. In MT1-MMP negative MCF-7 breast adenocarcinoma cells, both MT1-hIC7L and hIC7L showed no obvious fluorescence. In addition, the fluorescence intensity of C6 cells treated with MT1-hIC7L was suppressed by pre-treatment with an MT1-MMP endocytosis inhibitor (P < 0.05). In vivo optical imaging using probes intravenously administered to tumor-bearing mice showed that MT1-hIC7L specifically visualized C6 tumors (tumor-to-background ratios: 3.8 ± 0.3 [MT1-hIC7L] vs 3.1 ± 0.2 [hIC7L] 48 h after administration, P < 0.05), while the probes showed similarly low fluorescence in MCF-7 tumors. Together, these results show that MT1-hIC7L would be a potential activatable NIR probe for specifically detecting MT1-MMP-expressing tumors. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Wiley | en |
dc.rights | This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. | en |
dc.title | In vivo imaging of membrane type-1 matrix metalloproteinase with a novel activatable near-infrared fluorescence probe. | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Cancer science | en |
dc.identifier.volume | 105 | - |
dc.identifier.issue | 8 | - |
dc.identifier.spage | 1056 | - |
dc.identifier.epage | 1062 | - |
dc.relation.doi | 10.1111/cas.12457 | - |
dc.textversion | publisher | - |
dc.identifier.pmid | 24863849 | - |
dcterms.accessRights | open access | - |
出現コレクション: | 学術雑誌掲載論文等 |
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