ダウンロード数: 355

このアイテムのファイル:
ファイル 記述 サイズフォーマット 
j.stemcr.2014.07.005.pdf3.92 MBAdobe PDF見る/開く
完全メタデータレコード
DCフィールド言語
dc.contributor.authorGotoh, Shimpeien
dc.contributor.authorIto, Isaoen
dc.contributor.authorNagasaki, Tadaoen
dc.contributor.authorYamamoto, Yukien
dc.contributor.authorKonishi, Satoshien
dc.contributor.authorKorogi, Yoheien
dc.contributor.authorMatsumoto, Hisakoen
dc.contributor.authorMuro, Shigeoen
dc.contributor.authorHirai, Toyohiroen
dc.contributor.authorFunato, Michinorien
dc.contributor.authorMae, Shin-Ichien
dc.contributor.authorToyoda, Taroen
dc.contributor.authorSato-Otsubo, Aikoen
dc.contributor.authorOgawa, Seishien
dc.contributor.authorOsafune, Kenjien
dc.contributor.authorMishima, Michiakien
dc.contributor.alternative伊藤, 功朗ja
dc.contributor.alternative小川, 誠司ja
dc.contributor.alternative長船, 健二ja
dc.contributor.alternative三嶋, 理晃ja
dc.date.accessioned2014-10-02T05:32:06Z-
dc.date.available2014-10-02T05:32:06Z-
dc.date.issued2014-09-09-
dc.identifier.issn0092-8674-
dc.identifier.urihttp://hdl.handle.net/2433/190413-
dc.descriptionヒトiPS細胞から肺胞上皮細胞を分化誘導し、単離する方法を確立 -肺の再生/創薬研究につながる大きな一歩-. 京都大学プレスリリース. 2014-08-22.ja
dc.description.abstractNo methods for isolating induced alveolar epithelial progenitor cells (AEPCs) from human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) have been reported. Based on a study of the stepwise induction of alveolar epithelial cells (AECs), we identified carboxypeptidase M (CPM) as a surface marker of NKX2-1(+) "ventralized" anterior foregut endoderm cells (VAFECs) in vitro and in fetal human and murine lungs. Using SFTPC-GFP reporter hPSCs and a 3D coculture system with fetal human lung fibroblasts, we showed that CPM(+) cells isolated from VAFECs differentiate into AECs, demonstrating that CPM is a marker of AEPCs. Moreover, 3D coculture differentiation of CPM(+) cells formed spheroids with lamellar-body-like structures and an increased expression of surfactant proteins compared with 2D differentiation. Methods to induce and isolate AEPCs using CPM and consequently generate alveolar epithelial spheroids would aid human pulmonary disease modeling and regenerative medicine.en
dc.format.mimetypeapplication/pdf-
dc.language.isoeng-
dc.publisherElsevier Inc.en
dc.rightsThis is an open access article under the CC BY license (http://creativecommons.org/licenses/by/3.0/).en
dc.titleGeneration of Alveolar Epithelial Spheroids via Isolated Progenitor Cells from Human Pluripotent Stem Cells.en
dc.typejournal article-
dc.type.niitypeJournal Article-
dc.identifier.ncidAA00600003-
dc.identifier.jtitleStem cell reportsen
dc.identifier.volume3-
dc.identifier.issue3-
dc.identifier.spage394-
dc.identifier.epage403-
dc.relation.doi10.1016/j.stemcr.2014.07.005-
dc.textversionpublisher-
dc.identifier.pmid25241738-
dc.relation.urlhttps://www.kyoto-u.ac.jp/ja/research-news/2014-08-22-
dcterms.accessRightsopen access-
出現コレクション:学術雑誌掲載論文等

アイテムの簡略レコードを表示する

Export to RefWorks


出力フォーマット 


このリポジトリに保管されているアイテムはすべて著作権により保護されています。