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Title: Guard Cell Chloroplasts Are Essential for Blue Light-Dependent Stomatal Opening in Arabidopsis
Authors: Suetsugu, Noriyuki  kyouindb  KAKEN_id
Takami, Tsuneaki
Ebisu, Yuuta
Watanabe, Harutaka
Iiboshi, Chihoko
Doi, Michio
Shimazaki, Ken-ichiro
Author's alias: 末次, 憲之
島崎, 研一郎
Issue Date: 24-Sep-2014
Publisher: Public Library of Science
Journal title: PLoS ONE
Volume: 9
Issue: 9
Thesis number: e108374
Abstract: Blue light (BL) induces stomatal opening through the activation of H[+]-ATPases with subsequent ion accumulation in guard cells. In most plant species, red light (RL) enhances BL-dependent stomatal opening. This RL effect is attributable to the chloroplasts of guard cell, the only cells in the epidermis possessing this organelle. To clarify the role of chloroplasts in stomatal regulation, we investigated the effects of RL on BL-dependent stomatal opening in isolated epidermis, guard cell protoplasts, and intact leaves of Arabidopsis thaliana. In isolated epidermal tissues and intact leaves, weak BL superimposed on RL enhanced stomatal opening while BL alone was less effective. In guard cell protoplasts, RL enhanced BL-dependent H[+]-pumping and DCMU, a photosynthetic electron transport inhibitor, eliminated this effect. RL enhanced phosphorylation levels of the H[+]-ATPase in response to BL, but this RL effect was not suppressed by DCMU. Furthermore, DCMU inhibited both RL-induced and BL-dependent stomatal opening in intact leaves. The photosynthetic rate in leaves correlated positively with BL-dependent stomatal opening in the presence of DCMU. We conclude that guard cell chloroplasts provide ATP and/or reducing equivalents that fuel BL-dependent stomatal opening, and that they indirectly monitor photosynthetic CO{2} fixation in mesophyll chloroplasts by absorbing PAR in the epidermis.
Rights: © 2014 Suetsugu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
DOI(Published Version): 10.1371/journal.pone.0108374
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