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j.bbamcr.2014.10.004.pdf | 7.86 MB | Adobe PDF | 見る/開く | |
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タイトル: | An optical labeling-based proliferation assay system reveals the paracrine effect of interleukin-6 in breast cancer. |
著者: | Itou, Junji Tanaka, Sunao Sato, Fumiaki Akiyama, Ryutaro Kawakami, Yasuhiko Toi, Masakazu https://orcid.org/0000-0003-1488-9958 (unconfirmed) |
著者名の別形: | 伊東, 潤二 |
キーワード: | Breast cancer Interleukin-6 Optical labeling Paracrine effect Proliferation The OPA system |
発行日: | 12-Oct-2014 |
出版者: | Elsevier B.V. |
誌名: | Biochimica et biophysica acta |
巻: | 1853 |
号: | 1 |
開始ページ: | 27 |
終了ページ: | 40 |
抄録: | Proliferation analysis is one of the basic approaches to characterize various cell types. In conventional cell proliferation assays, the same sample cannot be observed over time, nor can a specific group within a heterogeneous population of cells, for example, cancerous cells, be analyzed separately. To overcome these limitations, we established an optical labeling-based proliferation assay system with the Kaede protein, whose fluorescence can be irreversibly photoconverted from green to red by irradiation. After a single non-toxic photoconversion event, the intensity of red fluorescence in each cell is reduced by cell division. From this, we developed a simple method to quantify cell proliferation by monitoring reduction of red fluorescence over time. This study shows that the optical labeling-based proliferation assay is a viable novel method to analyze cell proliferation, and could enhance our understanding of mechanisms regulating cell proliferation machinery. We used this newly established system to analyze the functions of secreted interleukin-6 (IL-6) in cancer cell proliferation, which had not been fully characterized. Reduction in proliferation was observed following IL-6 knockdown. However, after co-culturing with IL-6-expressing cells, the proliferation of Kaede-labeled IL-6-knockdown cells was restored. These data indicate that in basal-like breast cancer cells, IL-6 exhibits a paracrine effect to positively regulate cell proliferation. Our results thus demonstrate that cancer cells can secrete signaling molecules, such as IL-6, to support the proliferation of other cancer cells. |
著作権等: | © 2014 Elsevier B.V. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/191230 |
DOI(出版社版): | 10.1016/j.bbamcr.2014.10.004 |
PubMed ID: | 25305574 |
出現コレクション: | 学術雑誌掲載論文等 |
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