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Title: マツ属の交雑育種に関する研究 (II) : クロマツ×アカマツ他数種類の種間交雑における受精過程について
Other Titles: Studies on the cross-breeding of Genus Pinus (II) : On the process of fertilization in the interspecific pollination of Pinus thunbergii Parl. with P. densiflora Sieb. et Zucc. and other pine species
Authors: 中井, 勇  KAKEN_name
藤本, 博次  KAKEN_name
稲森, 幸雄  KAKEN_name
Author's alias: Nakai, Isamu
Fujimoto, Hirotsugu
Inamori, Yukio
Issue Date: 25-Oct-1976
Publisher: 京都大学農学部附属演習林
Journal title: 京都大学農学部演習林報告
Volume: 48
Start page: 31
End page: 45
Abstract: 本報告はPinus thunbergii Parl. を雌性親とし, Pinus densiflora Sieb. et Zucc. 他数種類の種間交雑を行ない, それぞれの受精過程を組織学的に調べ検討したものである。交雑は1968年京都大学農学部附属演習林上賀茂試験地で行なわれ, 調査は受粉時から受精時まで, 一定期間ごとに球果を採取固定し, 永久プレパラートを作成し, 胚珠の発達経過が検鏡された。その結果, P. thunbergii の種内, P. thunbergii×P. densiflora, ×P. massoniana, ×P. luchuensis の種間では雌, 雄性配偶体とも正常に発達し, 種子の成熟時における調査では充実種子が得られた。しかし, ×P. banksiana, ×P. pinaster, ×P. rigida, ×P. taedaなどの4組合せでは, 雌性配偶体の発達は正常であったが, 雄性配偶体の発達は不正常であり, このことが充実種子の得られない結果となった。充実種子の得られなかった組合せの不稔現象は, 受精過程の中で, もっとも早い時期に起り, 花粉の不発芽や花粉管の伸長がまったくみられなかった。この現象については種々考えられるが, とくに, 種類それぞれがもつ遺伝的要因, あるいは, 化学的物質などのちがいが, 花粉の発芽や伸長に対し, 制御反応として働くためであろうと考えられる。
This paper discusses the results of microscopic examination on the histological process of fertilization by the interspecific cross pollination in the combinations of Pinus thunbergii Parl. with P. densiflora Sieb. et Zucc. and several pine species. The work of pollination was carried out in 1968 at Kamigamo Experimental Forest Station of Kyoto University. In order to make clear the process in the development of ovule, the strobili were collected at definite intervals, then they were fixed by bouin's fluid. Ovules were cut to serial sections by rotary microtome and they were fixed as parmanent preparation by usual method. As the results of anatomical examination on the development of ovule, female and male gametophytes were normal to develop in the intraspecific cross pollination of P. thunbergii ×P. thunbergii as the control cross for this examination and in three interspecific pollinations of P. thunbergii×P. densiflora, ×P. massoniana Lamb., ×P. luchuensis Mayr.. Full seeds were obtained by these intra- and interspecific pollinations, when the seeds matured. It, therefore, means that both developments of male and female gametophytes have been normal. By these pollinations in which male and female gametophytes developed nomally, it was possible to grasp principal tendency toward the stage in the development of ovule, but with a little early or late among samples. It was, namely, observed that the endosperm was formed within late May to early June at about 400 days after pollination, then the archegonia was formed. And the egg cell was examined in late June at about 410 days after pollination. On the other hand, the path of the pollen tube through the nucellus was observed in late June at about 415 days after pollination and the pollen tube invated into the archegonia in late June, so that the fertilization would be seen soon. Contrary to above-mentioned, other four interspecific pollinations in the combinations of P. thunbergii×P. banksiana Lamb., ×P. pinaster Ait., ×P. rigida Mill. and ×P. taeda Linn. did not produce any full seed. The failure in such combinations signifies that a development of male gametophyte within the term from pollinaton to fertilization was abnormal, despite a development of female gametophyte was normal. A sterile phenomenon in these pollinations, neither a pollen could germinate nor a pollen tube could elongate, was observed at very early time at one month after pollination. Although there will be various inferences in a reason of causing this sterile phenomenon, it seems that some genetic factors of specific characters or chemical difference between ovules of these species may be related to a control reaction for the germination of pollen or the elongation of pollen tube.
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