Deposition Process of Polysaccharides with the Development of Japanese Walnut Xylem

Abstract

オニグルミの木部形成にともなう木材多糖の絶対量の変化を薄層クロマトグラフィー・デンシトメトリーで調べた。すなわち分化中木部を形成層側から髄側へ順次分け取り, 種々の抽出処理をし酸加水分解した後, 中和・濃縮し, 薄層クロマトグラフィーで糖を分離した。そしてエチレンジアミン硫酸塩を噴霧し各糖のスポットの蛍光の強さを測定して定量した。セルロースはS_1形成期からS_2形成期まで継続的に堆積する。グルクロノキシランもセルロースとほぼ同様な堆積の仕方を示す。グルコマンナンはセルロースやグルクロノキシランの堆積よりもやや遅れ, S_2形成後期からS_3形成期にかけて堆積する。アラビノースやガラクトースは一次壁形成期に堆積するが二次壁形成期には壁に供給されないものと思われる。

Thin-layer-chromatography densitometry was applied to investigate the changes of the absolute amount of sugar with the xylem development of Japanese walnut (Juglans Sieboldiana Maxim.). The differentiating xylem was successively fractionated from the cambium side to the pith side. Each fraction was extracted with reagents to remove lipids and starch. After hydrolyzing each fraction, the hydrolysates were separated by thin-layer-chromatography. Aqueous solution of ethylenediamine sulfate was sprayed on the TLC-plate, and the fluorescent light of each spot in each fraction was measured by TLC-scanner. Cellulose deposits continuously from the S1 stage to the S3 stage. Glucuronoxylan deposits in the same manner as cellulose, although the amount of the former is less than that of the latter. On the other hand, the deposition of glucomannan occurs behind the cellulose and glucuronoxylan depositions, that is, it occurs from the later part of S2 stage to the S3 stage. Arabinose and galactose do not deposit during the secondary wall formation stage, although they deposit during the primary wall formation stage.