Induction of Callus from Mistletoe and Interaction with its Host Cells

Abstract

ヤドリギ (Viscum album L. var lutescens M.) から、カイネチン1. 0ppm, ナフタレン酢酸10. 0ppmを含む, イオン強度を半分にしたMurashige-Skoog (MS) 改変培地を用い, 28℃、12時間の明暗周期, 光度約2, 5001uxの条件下で培養し, カルスを誘導した。カルスは淡いピンク色で, その細胞の平均直径は約7. 5μmであった。また, プランテックスを用いて, 3週間培養したとき, その成長量が最大に達し, 絶乾重量が約3倍になった。カルス細胞には, 分子量77, 000、19, 400のガラク卜ース特異性レクチンが見出されたが, これらは, ヤドリギの葉にも同様に見出された。一方、ヤドリギのカルスをブナのカルスに接触させたところ両カルスは接触阻害を起さずに生長し続けることがわかった。

A callus was induced from mistletoe (Viscum album L. var lutescens M.), and established on a modified Murashige-Skoog (MS) medium having a half of the ionic strengh of the original MS medium and supplemented with 1.0 ppm of kinetin and 10.0 ppm of 1- naphthaleneacetic acid at 28℃ under 12-hr day-length at about 2, 500 lux. The callus was creamish pale pink and mainly spherical having average diameter of 7.5 μm. The growth of the callus reached a maximum at 3 weeks incubation in PLANTEX to give about thrice amount of dry weight. The callus contained two galactose-binding lectins having molecular weights of 77, 000 and 19, 400 which were originally observed in the leaves of mistletoe. When the callus of mistletoe was in contact with its host beech callus, both calluses were found to grow without contact inhibition.