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dc.contributor.authorSerizawa, Yasuhiroja
dc.contributor.authorOshima, Riekoja
dc.contributor.authorYoshida, Mitsukija
dc.contributor.authorSakon, Ichikaja
dc.contributor.authorKitani, Kazutoja
dc.contributor.authorGoto, Ayumija
dc.contributor.authorTsuda, Satoshija
dc.contributor.authorHayashi, Tatsuyaja
dc.contributor.alternative大島, 里詠子ja
dc.description.abstractSalicylate (SAL) has been recently implicated in the antidiabetic effect in humans. We assessed whether 5'-AMP-activated protein kinase (AMPK) in skeletal muscle is involved in the effect of SAL on glucose homeostasis. Rat fast-twitch epitrochlearis and slow-twitch soleus muscles were incubated in buffer containing SAL. Intracellular concentrations of SAL increased rapidly (<5min) in both skeletal muscles, and the Thr(172) phosphorylation of the α subunit of AMPK increased in a dose- and time-dependent manner. SAL increased both AMPKα1 and AMPKα2 activities. These increases in enzyme activity were accompanied by an increase in the activity of 3-O-methyl-d-glucose transport, and decreases in ATP, phosphocreatine, and glycogen contents. SAL did not change the phosphorylation of insulin receptor signaling including insulin receptor substrate 1, Akt, and p70 ribosomal protein S6 kinase. These results suggest that SAL may be transported into skeletal muscle and may stimulate AMPK and glucose transport via energy deprivation in multiple muscle types. Skeletal muscle AMPK might be part of the mechanism responsible for the metabolic improvement induced by SAL.ja
dc.publisherElsevier Inc.ja
dc.rights© 2014 Elsevier Inc.ja
dc.rightsThis is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。ja
dc.subject5′-AMP-activated protein kinaseja
dc.subjectGlucose transportja
dc.subjectInsulin signalingja
dc.subjectSkeletal muscleja
dc.subjectDiabetes mellitusja
dc.titleSalicylate acutely stimulates 5'-AMP-activated protein kinase and insulin-independent glucose transport in rat skeletal muscles.ja
dc.type.niitypeJournal Articleja
dc.identifier.jtitleBiochemical and biophysical research communicationsja
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