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Title: The unexpected role of polyubiquitin chains in the formation of fibrillar aggregates
Authors: Morimoto, Daichi  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-7672-2136 (unconfirmed)
Walinda, Erik  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0003-1882-6401 (unconfirmed)
Fukada, Harumi
Sou, Yu-Shin
Kageyama, Shun
Hoshino, Masaru  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0003-4099-0232 (unconfirmed)
Fujii, Takashi
Tsuchiya, Hikaru
Saeki, Yasushi
Arita, Kyohei
Ariyoshi, Mariko
Tochio, Hidehito  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0003-3843-3330 (unconfirmed)
Iwai, Kazuhiro  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0001-5620-5951 (unconfirmed)
Namba, Keiichi
Komatsu, Masaaki
Tanaka, Keiji
Shirakawa, Masahiro
Author's alias: 森本, 大智
白川, 昌宏
Keywords: Biological sciences
Biochemistry
Cell biology
Issue Date: 20-Jan-2015
Publisher: Nature Publishing Group
Journal title: Nature Communications
Volume: 6
Thesis number: 6116
Abstract: Ubiquitin is known to be one of the most soluble and stably folded intracellular proteins, but it is often found in inclusion bodies associated with various diseases including neurodegenerative disorders and cancer. To gain insight into this contradictory behaviour, we have examined the physicochemical properties of ubiquitin and its polymeric chains that lead to aggregate formation. We find that the folding stability of ubiquitin chains unexpectedly decreases with increasing chain length, resulting in the formation of amyloid-like fibrils. Furthermore, when expressed in cells, polyubiquitin chains covalently linked to EGFP also form aggregates depending on chain length. Notably, these aggregates are selectively degraded by autophagy. We propose a novel model in which the physical and chemical instability of polyubiquitin chains drives the formation of fibrils, which then serve as an initiation signal for autophagy.
Description: ポリユビキチン鎖のアミロイド様線維形成を発見 -神経変性疾患における脳内異常タンパク質凝集の形成機構解明に期待-. 京都大学プレスリリース. 2015-01-21.
Rights: This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
URI: http://hdl.handle.net/2433/193230
DOI(Published Version): 10.1038/ncomms7116
Related Link: http://www.kyoto-u.ac.jp/ja/research/research_results/2014/150120_1.html
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