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タイトル: | Acquisition of the Ability To Assimilate Mannitol by Saccharomyces cerevisiae through Dysfunction of the General Corepressor Tup1-Cyc8. |
著者: | Chujo, Moeko Yoshida, Shiori Ota, Anri Murata, Kousaku Kawai, Shigeyuki |
著者名の別形: | 河井, 重幸 |
発行日: | 1-Jan-2015 |
出版者: | American Society for Microbiology |
誌名: | Applied and environmental microbiology |
巻: | 81 |
号: | 1 |
開始ページ: | 9 |
終了ページ: | 16 |
抄録: | Saccharomyces cerevisiae normally cannot assimilate mannitol, a promising brown macroalgal carbon source for bioethanol production. The molecular basis of this inability remains unknown. We found that cells capable of assimilating mannitol arose spontaneously from wild-type S. cerevisiae during prolonged culture in mannitol-containing medium. Based on microarray data, complementation analysis, and cell growth data, we demonstrated that acquisition of mannitol-assimilating ability was due to spontaneous mutations in the genes encoding Tup1 or Cyc8, which constitute a general corepressor complex that regulates many kinds of genes. We also showed that an S. cerevisiae strain carrying a mutant allele of CYC8 exhibited superior salt tolerance relative to other ethanologenic microorganisms; this characteristic would be highly beneficial for the production of bioethanol from marine biomass. Thus, we succeeded in conferring the ability to assimilate mannitol on S. cerevisiae through dysfunction of Tup1-Cyc8, facilitating production of ethanol from mannitol. |
著作権等: | Copyright © American Society for Microbiology, 'Applied and Environmental Microbiology',vol.81, 2015,pp.9-16,doi:10.1128/AEM.02906-14. |
URI: | http://hdl.handle.net/2433/193286 |
DOI(出版社版): | 10.1128/AEM.02906-14 |
PubMed ID: | 25304510 |
出現コレクション: | 学術雑誌掲載論文等 |
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