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dc.contributor.author | Takashima, Seiji | en |
dc.contributor.author | Kanatsu-Shinohara, Mito | en |
dc.contributor.author | Tanaka, Takashi | en |
dc.contributor.author | Morimoto, Hiroko | en |
dc.contributor.author | Inoue, Kimiko | en |
dc.contributor.author | Ogonuki, Narumi | en |
dc.contributor.author | Jijiwa, Mayumi | en |
dc.contributor.author | Takahashi, Masahide | en |
dc.contributor.author | Ogura, Atsuo | en |
dc.contributor.author | Shinohara, Takashi | en |
dc.contributor.alternative | 高島, 誠司 | ja |
dc.contributor.alternative | 篠原, 隆司 | ja |
dc.date.accessioned | 2015-02-16T01:16:23Z | - |
dc.date.available | 2015-02-16T01:16:23Z | - |
dc.date.issued | 2015-02 | - |
dc.identifier.issn | 2213-6711 | - |
dc.identifier.uri | http://hdl.handle.net/2433/193679 | - |
dc.description | 精子幹細胞の新しい自己複製様式の発見 -男性不妊症の原因解明、遺伝病の発症機序の理解に期待-. 京都大学プレスリリース. 2015-02-13. | ja |
dc.description.abstract | Spermatogonial stem cells (SSCs) are required for spermatogenesis. Earlier studies showed that glial cell line-derived neurotrophic factor (GDNF) was indispensable for SSC self-renewal by binding to the GFRA1/RET receptor. Mice with mutations in these molecules showed impaired spermatogenesis, which was attributed to SSC depletion. Here we show that SSCs undergo GDNF-independent self-renewal. A small number of spermatogonia formed colonies when testis fragments from a Ret mutant mouse strain were transplanted into heterologous recipients. Moreover, fibroblast growth factor 2 (FGF2) supplementation enabled in vitro SSC expansion without GDNF. Although GDNF-mediated self-renewal signaling required both AKT and MAP2K1/2, the latter was dispensable in FGF2-mediated self-renewal. FGF2-depleted testes exhibited increased levels of GDNF and were enriched for SSCs, suggesting that the balance between FGF2 and GDNF levels influences SSC self-renewal in vivo. Our results show that SSCs exhibit at least two modes of self-renewal and suggest complexity of SSC regulation in vivo. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier Inc. | en |
dc.rights | ©2015 The Authors. | en |
dc.rights | This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). | en |
dc.title | Functional Differences between GDNF-Dependent and FGF2-Dependent Mouse Spermatogonial Stem Cell Self-Renewal | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Stem Cell Reports | en |
dc.identifier.volume | 4 | - |
dc.identifier.issue | 3 | - |
dc.identifier.spage | 489 | - |
dc.identifier.epage | 502 | - |
dc.relation.doi | 10.1016/j.stemcr.2015.01.010 | - |
dc.textversion | publisher | - |
dc.identifier.pmid | 25684228 | - |
dc.relation.url | https://www.kyoto-u.ac.jp/ja/research-news/2015-02-13 | - |
dcterms.accessRights | open access | - |
出現コレクション: | 学術雑誌掲載論文等 |
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