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タイトル: | Different correlations among physiological and morphological properties at single glutamatergic synapses in the rat hippocampus and the cerebellum. |
著者: | Miyawaki, Hiroyuki Hirano, Tomoo |
著者名の別形: | 平野, 丈夫 |
キーワード: | quantal EPSC presynaptic varicosity spine culture |
発行日: | 20-Oct-2010 |
出版者: | wiley |
誌名: | Synapse |
巻: | 65 |
号: | 5 |
開始ページ: | 412 |
終了ページ: | 423 |
抄録: | Synapses in the mammalian central nervous system show substantial diversity in their physiological and morphological properties. However, the correlations among them have remained elusive. Here, we tried to clarify the correlations by establishing a method to record excitatory postsynaptic currents (EPSCs) at individual synapses and also to observe the morphology at the same time. A pair of pre- and postsynaptic neurons were labeled with different fluorescent dyes, and a presynaptic varicosity was selectively stimulated with a θ-tube glass electrode under conditions in which action potential generation was suppressed. Two representative types of excitatory glutamatergic synapses, one on hippocampal pyramidal neurons and the other on cerebellar Purkinje neurons, were studied. The correlations between the properties of quantal EPSCs (qEPSCs) and those of synaptic morphology were analyzed in rat primary culture preparations. The amplitude and the decay time of qEPSC were correlated with the size of the postsynaptic spine only at hippocampal synapses. In contrast, the size of the presynaptic varicosity was correlated with the size of the postsynaptic spine and the quantal content of evoked EPSCs only at granule neuron-Purkinje neuron synapses in the cerebellum. These results suggest that the interaction between pre- and postsynaptic structures and the coupling of postsynaptic responsiveness and the spine morphology differ between cerebellar and hippocampal glutamatergic synapses. |
著作権等: | This is the peer reviewed version of the following article: Miyawaki, H. and Hirano, T. (2011), Different correlations among physiological and morphological properties at single glutamatergic synapses in the rat hippocampus and the cerebellum. Synapse, 65: 412–423, which has been published in final form at http://dx.doi.org/10.1002/syn.20860 この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/197147 |
DOI(出版社版): | 10.1002/syn.20860 |
PubMed ID: | 20812293 |
出現コレクション: | 学術雑誌掲載論文等 |
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