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タイトル: | Role of endogenous TGF-β family in myogenic differentiation of C2C12 cells. |
著者: | Furutani, Yuuma Umemoto, Takenao Murakami, Masaru Matsui, Tohru Funaba, Masayuki https://orcid.org/0000-0002-4303-6682 (unconfirmed) |
著者名の別形: | 舟場, 正幸 |
キーワード: | TGF-β family BMP TGF-β myotube differentiation |
発行日: | 25-Jan-2011 |
出版者: | wiley |
誌名: | Journal of cellular biochemistry |
巻: | 112 |
号: | 2 |
開始ページ: | 614 |
終了ページ: | 624 |
抄録: | The present study evaluated endogenous activities and the role of BMP and transforming growth factor-β (TGF-β), representative members of the TGF-β family, during myotube differentiation in C2C12 cells. Smad phosphorylation at the C-terminal serines was monitored, since TGF-β family members signal via the phosphorylation of Smads in a ligand-dependent manner. Expression of phosphorylated Smad1/5/8, which is an indicator of BMP activity, was higher before differentiation, and rapidly decreased after differentiation stimulation. Differentiation-related changes were consistent with those in the expression of Ids, well-known BMP-responsive genes. Treatment with inhibitors of BMP type I receptors or noggin in C2C12 myoblasts down-regulated the expression of myogenic regulatory factors, such as Myf5 and MyoD, leading to impaired myotube formation. Addition of BMP-2 during the myoblast phase also inhibited myotube differentiation through the down-regulation of Myf5 and MyoD. In contrast to endogenous BMP activity, the phosphorylation of Smad2, a TGF-β-responsive Smad, was higher 8-16 days after differentiation stimulation. A-83-01, an inhibitor of TGF-β type I receptor, increased the expression of Myf5 and MyoD, and enhanced myotube formation. The present results reveal that endogenous activities of the TGF-β family are changed during myogenesis in a pathway-specific manner, and that the activities are required for myogenesis. |
著作権等: | This is the peer reviewed version of the following article: Furutani, Y., Umemoto, T., Murakami, M., Matsui, T. and Funaba, M. (2011), Role of endogenous TGF-β family in myogenic differentiation of C2C12 cells. J. Cell. Biochem., 112: 614–624, which has been published in final form at http://dx.doi.org/10.1002/jcb.22953 この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/197160 |
DOI(出版社版): | 10.1002/jcb.22953 |
PubMed ID: | 21268083 |
出現コレクション: | 学術雑誌掲載論文等 |
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